alpha Macroglobulin Mouse ELISA Kit (ab108887)
- Product namealpha Macroglobulin Mouse ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 5%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Urine, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity= 0.07 ng/ml
- Range0.078 ng/ml - 20 ng/ml
- Assay time4h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Mouse
- Product overview
Abcam’s alpha Macroglobulin Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Macroglobulin levels in plasma, serum, urine, and cell culture supernatants.
An alpha Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Macroglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Macroglobulin captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated alpha Macroglobulin Antibody 1 x 140µl alpha Macroglobulin Microplate (12 x 8 well strips) 1 unit alpha Macroglobulin Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml
- Research Areas
- SwissProt: Q61838 Mouse
Our Abpromise guarantee covers the use of ab108887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for alpha Macroglobulin Mouse ELISA Kit (ab108887)
ab108887 has not yet been referenced specifically in any publications.