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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Glucosaminoglycans
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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - Anti-alpha Sarcoglycan antibody [Ad1/20A6] (ab49451)](/ps/datasheet/images/49/ab49451/alpha-Sarcoglycan-Primary-antibodies-ab49451-4.jpg)
Western blot - Anti-alpha Sarcoglycan antibody [Ad1/20A6] (ab49451)
Anti-alpha Sarcoglycan antibody [Ad1/20A6] (ab49451) at 1/250 dilution +
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
Alpha Sarcoglycan contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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