|
|
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Synuclein antibody [4B12] (ab1904)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Synuclein antibody [4B12] (ab1904)](/ps/datasheet/images/ab1904_1.jpg)
(enlarge)
|
|
Western blot - alpha Synuclein antibody [4B12] (ab1904)
![Western blot - alpha Synuclein antibody [4B12] (ab1904)](/ps/datasheet/images/ab1904_2.jpg)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Synuclein antibody [4B12] (ab1904)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Synuclein antibody [4B12] (ab1904)](/ps/datasheet/images/ab1904_3.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
alpha Synuclein antibody [4B12]
|
| |
See all alpha Synuclein antibodies (28)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [4B12] to alpha Synuclein
|
|
Immunogen
|
Recombinant human alpha synuclein produced in E. coli.
|
|
Reacts with
|
Hu
|
|
Does not react with
(species key)
|
Ms, Rat
|
|
Tested applications
(see key)
|
Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
Dot: 1/100 - 1/10000.
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: 1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.
IHC-Fr: 1/100 - 1/1000.
WB: 1/100 - 1/10000. Predicted molecular weight: 16 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasm. Membrane. Nucleus. Membrane-bound in dopaminergic neurons. Also found in the nucleus.
|
|
Research areas
|
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Synuclein
|
|
Relevance
|
Alpha Synuclein is implicated in the regulation of dopamine release and transport. It is a soluble protein, expressed principally in the brain but also expressed in low concentrations in all tissues examined (except liver). In the nervous system, alpha Synuclein is primarily located at presynaptic terminals and is found membrane bound in dopaminergic neurons. It can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies), including Parkinson's Disease. Alpha Synuclein induces fibrillization of microtubule associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. Alpha synuclein is a protein phosphorylated predominantly on serine residues.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
4B12
|
|
Isotype
|
IgG1
|
|
Storage buffer
|
Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Ammonium sulfate precipitated and dialyzed tissue culture supernatant.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this alpha Synuclein antibody [4B12] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for alpha Synuclein antibody [4B12] images, references, products related to ab1904 and other tools.
|
| |
alpha Synuclein antibody [4B12] images:
|
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Synuclein antibody [4B12] (ab1904)
IHC-P using ab1904 showing cortical lewy body (x400).
|
| |
Western blot - alpha Synuclein antibody [4B12] (ab1904)
Predicted band size : 16 kDa
Western blot using ab1904 as detection of 20 µg of human neuroblastoma cell lysate (20 µg) overexpressing human a-synuclein (1) and recombinant a-synuclein (2) (50 ng).
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Synuclein antibody [4B12] (ab1904)
IHC-P using ab1904 showing lewy body of a substantia nigra neuron from a parkinson’s disease patient.
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Synuclein antibody [4B12] (ab1904)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Synuclein antibody [4B12] (ab1904)](/ps/datasheet/images/ab1904_4.jpg)
IHC-P using ab1904. Synuclein-positive dystrophic neurites in the CA2 sector of the hippocampus. Diffuse Lewy body disease. B, C: Orig. magn. X 200
|
| |
Immunocytochemistry/ Immunofluorescence - alpha Synuclein antibody [4B12] (ab1904)
![Immunocytochemistry/ Immunofluorescence - alpha Synuclein antibody [4B12] (ab1904)](\ps\datasheet\images\1\ab1904\alpha-Synuclein-Primary-antibodies-ab1904-2.jpg)
ICC/IF image of ab1904 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1904, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
|
| |
References for alpha Synuclein antibody [4B12] (ab1904)
|
|
This product has been used in: (two most recent references)
Fountaine TM et al. The effect of alpha-synuclein knockdown on MPP+ toxicity in models of human neurons. Eur J Neurosci 28:2459-73 (2008). WB, ICC/IF; Human. PubMed: 19032594
Boyer F & Dreyer JL Alpha-synuclein in the nucleus accumbens induces changes in cocaine behaviour in rats. Eur J Neurosci 26:2764-76 (2007). PubMed: 18001274
If you publish research using ab1904 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to alpha Synuclein
|
| |
alpha Synuclein antibody [4B12] - more information
|
Customer reviews (feedback) regarding alpha Synuclein antibody [4B12] |
Customer FAQs regarding alpha Synuclein antibody [4B12] |
Protocols for alpha Synuclein antibody [4B12] |
Price and availability of products related to alpha Synuclein antibody [4B12] |
| |
alpha Synuclein antibody [4B12] - related products:
|
|
|
| |
alpha Synuclein antibody [4B12] - other tools:
|
Contact Abcam with a Technical Enquiry about alpha Synuclein antibody [4B12] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Synuclein antibody [4B12] (ab1904)](/index.html?datasheet=1904#ab1904_1.jpg)
![Western blot - alpha Synuclein antibody [4B12] (ab1904)](/index.html?datasheet=1904#ab1904_2.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Synuclein antibody [4B12] (ab1904)](/index.html?datasheet=1904#ab1904_3.jpg)
