Anti-Alpha-synuclein antibody (ab190376)
Key features and details
- Chicken polyclonal to Alpha-synuclein
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat
- Isotype: IgY
Overview
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Product name
Anti-Alpha-synuclein antibody
See all Alpha-synuclein primary antibodies -
Description
Chicken polyclonal to Alpha-synuclein -
Host species
Chicken -
Specificity
Due to high sequence homology ab190376 might react with Beta synuclein -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Recombinant full length protein corresponding to Human Alpha-synuclein aa 1 to the C-terminus. Epitope: amino acids 61-95.
Database link: P37840 -
Positive control
- IHC-P: Human and rat cerebellum tissue. WB: Rat brain crude extract. ICC/IF: Mixed rat neuron-glial cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.0325% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000.
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WB |
1/2000. Predicted molecular weight: 14 kDa.
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IHC-P |
1/3000 - 1/5000.
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Notes |
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ICC/IF
1/1000. |
WB
1/2000. Predicted molecular weight: 14 kDa. |
IHC-P
1/3000 - 1/5000. |
Target
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Function
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
Tissue specificity
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
Involvement in disease
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
Sequence similarities
Belongs to the synuclein family. -
Domain
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
Post-translational
modificationsPhosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
Cellular localization
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
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Database links
- Entrez Gene: 461375 Chimpanzee
- Entrez Gene: 102119369 Cynomolgus monkey
- Entrez Gene: 101146366 Gorilla
- Entrez Gene: 100190854 Orangutan
- Entrez Gene: 29219 Rat
- Entrez Gene: 706985 Rhesus monkey
- SwissProt: P61145 Chimpanzee
- SwissProt: 61142 Cynomolgus monkey
see all -
Alternative names
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
Images
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Chromogenic immunostaining of a formalin fixed paraffin embedded human cerebellum section with chicken pAb to α-synuclein, dilution 1/5000, detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain. The α-synuclein protein is concentrated in synapses, in particular highlighting the glomeruli synapses in the granule layer and those on Purkinje dendrites in the molecular layer.
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Anti-Alpha-synuclein antibody (ab190376) at 1/2000 dilution + Rat brain crude extract
Predicted band size: 14 kDa -
Immunofluorescent analysis of Mixed rat neuron-glial cultures labeling alpha Synuclein with ab190376 at 1/1000 dilution (red) and and monoclonal antibody to MAP2 (green); DNA (blue).
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Ab190376 staining alpha-synuclein in rat cerebellum tissue sections at 1/3000 dilution (red) and co-stained with rabbit polyclonal antibody to GFAP. DAPI was used as a nuclear counterstain.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab190376 has been referenced in 1 publication.
- Ren M et al. MED13 and glycolysis are conserved modifiers of α-synuclein-associated neurodegeneration. Cell Rep 41:111852 (2022). PubMed: 36543134