Anti-alpha Tubulin antibody [2Q467] (ab64332)
- Product nameAnti-alpha Tubulin antibody [2Q467]See all alpha Tubulin primary antibodies ...
- DescriptionRat monoclonal [2Q467] to alpha Tubulin
- SpecificityThe antibody has a greater affinity for formaldehyde or glutaraldehyde fixed microtubules than native.
- Tested applicationsWB, ELISA, IHC-P, IHC-Fr more details
- Species reactivityReacts with: Human
Predicted to work with: a wide range of other species, all Mammals
Full length protein (purified) (Human)
- Epitopeab64332 recognizes amino acids 414-422 of alpha Tubulin.
- Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 10mM Sodium Azide
Constituents: PBS, 1mg/ml BSA, pH 7.2
- Concentration information loading...
- PurityIon Exchange Chromatography
- Clonality Monoclonal
- Clone number2Q467
- Research Areas
Our Abpromise guarantee covers the use of ab64332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.|
|ELISA||ELISA: Use at an assay dependent dilution.|
|IHC-P||IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||IHC-Fr: Use at an assay dependent dilution.|
- FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
- Sequence similaritiesBelongs to the tubulin family.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
- Cellular localizationCytoplasm > cytoskeleton.
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- B ALPHA 1 antibody
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- H2 ALPHA antibody
- Hum a tub1 antibody
- Hum a tub2 antibody
- LIS3 antibody
- TBA4A_HUMAN antibody
- Testis-specific alpha-tubulin antibody
- TUBA1 antibody
- TUBA1A antibody
- Tuba4a antibody
- Tubulin alpha 1 chain antibody
- Tubulin alpha antibody
- Tubulin alpha ubiquitous chain antibody
- Tubulin alpha-1 chain antibody
- Tubulin alpha-4A chain antibody
- Tubulin H2-alpha antibody
- Tubulin K alpha 1 antibody
- Tubulin, alpha 1 (testis specific) antibody
- Tubulin, alpha 4a antibody
- Tubulin, alpha, testis-specific antibody
- Tubulin, alpha-1 antibody
References for Anti-alpha Tubulin antibody [2Q467] (ab64332)
ab64332 has not yet been referenced specifically in any publications.