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Overview

  • Product nameAnti-alpha Tubulin antibody [4G1]See all alpha Tubulin primary antibodies ...
  • Description
    Mouse monoclonal [4G1] to alpha Tubulin
  • Tested applicationsELISA, WB, Flow Cyt, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to C terminal amino acids of Human alpha Tubulin, conjugated to KLH

  • Positive controlSH-SY5Y cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab28439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use at an assay dependent concentration.
WB WB: 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
Flow Cyt Flow Cyt: Use 1µg for 106 cells.
ICC/IF ICC/IF: Use a concentration of 10 µg/ml.

Target

  • Alternative names
      Alpha tubulin 2 antibodyAlpha tubulin 3 antibodyAlpha tubulin 3C/D antibody
      Alpha tubulin ubiquitous antibodyB ALPHA 1 antibodybA408E5.3 antibodyH2 ALPHA antibodyHum a tub1 antibodyHum a tub2 antibodyLIS3 antibodyTUBA1 antibodyTUBA1A antibodyTUBA2 antibodyTUBA3 antibodyTUBA3C antibodyTUBA3D antibodyTUBA4A antibodyTubulin alpha 1 chain antibodyTubulin alpha 1A chain antibodyTubulin alpha 2 chain antibodyTubulin alpha 3 chain antibodyTubulin alpha 3C/D chain antibodyTubulin alpha antibodyTubulin alpha ubiquitous chain antibodyTubulin B alpha 1 antibodyTubulin K alpha 1 antibodyTubulin, alpha 1 (testis specific) antibodyTubulin, alpha 1a antibodyTubulin, alpha 2 antibodyTubulin, alpha 3 antibodyTubulin, alpha 3c antibodyTubulin, alpha 4a antibodyTubulin, alpha, brain specific antibody
    see all

Anti-alpha Tubulin antibody [4G1] images

  • ab28439 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab28439 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (100%) Hek293 and MCF-7 cells, and also in formaldehyde fixed (4%) HeLa, Hek293, HepG2, and MCF-7 cells at 10ug/ml.

  • All lanes : Anti-alpha Tubulin antibody [4G1] (ab28439) at 1/2000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : Mouse brain tissue
    Lane 3 : Rat brain tissue


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Overlay histogram showing HeLa cells stained with ab28439 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28439, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-alpha Tubulin antibody [4G1] (ab28439)

This product has been referenced in:
  • Hao J  et al. Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells. PLoS One 3:e2904 (2008). WB ; Mouse . Read more (PubMed: 18682835) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"