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Synthetic peptide corresponding to C terminal amino acids of Human alpha Tubulin, conjugated to KLH
Our Abpromise guarantee covers the use of ab28439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 54 kDa.|
|Flow Cyt||Use 1µg for 106 cells.|
|ICC/IF||Use a concentration of 10 µg/ml.|
ab28439 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab28439 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (100%) Hek293 and MCF-7 cells, and also in formaldehyde fixed (4%) HeLa, Hek293, HepG2, and MCF-7 cells at 10ug/ml.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"