![Western blot - alpha Tubulin antibody [4G1] (ab28439) image](#ab28439_1.JPG)
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Product name
Anti-alpha Tubulin antibody [4G1]
See all alpha Tubulin products (27) ...
Description
Mouse monoclonal [4G1] to alpha Tubulin
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to C terminal amino acids of Human alpha Tubulin, conjugated to KLH
Positive control
SH-SY5Y cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
4G1
Isotype
IgG1
Light chain type
kappa
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
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Western blot - alpha Tubulin antibody [4G1] (ab28439)
![Western blot - alpha Tubulin antibody [4G1] (ab28439) image](/ps/datasheet/Images/28/ab28439/ab28439_1.JPG)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
Target
Relevance
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
Cellular localization
Cytoplasmic
Alternative names
- Alpha tubulin 2 antibody
- Alpha tubulin 3 antibody
- Alpha tubulin 3C/D antibody
see all
Database links
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 7846 Human
- Entrez Gene: 22142 Mouse
- Entrez Gene: 64158 Rat
- Omim: 191110 Human
- Omim: 602529 Human
- Omim: 602528 Human
see all
Anti-alpha Tubulin antibody [4G1] images:
Western blot - alpha Tubulin antibody [4G1] (ab28439)
All lanes : Anti-alpha Tubulin antibody [4G1] (ab28439) at 1/2000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Mouse brain tissue
Lane 3 : Rat brain tissue
Predicted band size : 54 kDa
Observed band size : 54 kDa
References for Anti-alpha Tubulin antibody [4G1] (ab28439)
This product has been referenced in:
- Hao J et al. Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells. PLoS One 3:e2904 (2008). WB; Mouse.Read more (PubMed: 18682835) »
See 1 publication for this product
Publishing research using ab28439? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"