Anti-alpha Tubulin antibody [AA13] (ab28037)
- Product nameAnti-alpha Tubulin antibody [AA13]See all alpha Tubulin primary antibodies ...
- DescriptionMouse monoclonal [AA13] to alpha Tubulin
- SpecificityThis antibody will recognize rat brain tubulin and all alpha tubulin isotypes expressed in mammalian brain.
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
Rat brain tubulin.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Concentration information loading...
- PurityProtein A purified
- Clonality Monoclonal
- Clone numberAA13
- Research Areas
Our Abpromise guarantee covers the use of ab28037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/1000. Predicted molecular weight: 55 kDa.|
|ICC/IF||ICC/IF: Use at an assay dependent dilution.|
- FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
- Sequence similaritiesBelongs to the tubulin family.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
- Cellular localizationCytoplasm > cytoskeleton.
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- H2 ALPHA antibody
- Hum a tub1 antibody
- Hum a tub2 antibody
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- Testis-specific alpha-tubulin antibody
- TUBA1 antibody
- TUBA1A antibody
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- Tubulin alpha 1 chain antibody
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- Tubulin alpha ubiquitous chain antibody
- Tubulin alpha-1 chain antibody
- Tubulin alpha-4A chain antibody
- Tubulin H2-alpha antibody
- Tubulin K alpha 1 antibody
- Tubulin, alpha 1 (testis specific) antibody
- Tubulin, alpha 4a antibody
- Tubulin, alpha, testis-specific antibody
- Tubulin, alpha-1 antibody
References for Anti-alpha Tubulin antibody [AA13] (ab28037)
This product has been referenced in:
- Baldo B et al. A screen for enhancers of clearance identifies huntingtin as a heat shock protein 90 (hsp90) client protein. J Biol Chem 287:1406-14 (2012). WB . Read more (PubMed: 22123826) »
- Shimshek DR et al. Excess a-synuclein worsens disease in mice lacking ubiquitin carboxy-terminal hydrolase L1. Sci Rep 2:262 (2012). WB ; Mouse . Read more (PubMed: 22355774) »