Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
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ab11304 has been referenced in 8 publications.
Publishing research using ab11304? Please let us know so that we can cite the reference in this datasheet
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ab11304 at a 1/100 dilution staining human HeLa cells by Immunocytochemistry/Immunofluorescence. The cells were methanol fixed and blocked for 1 hour with 10% serum. The antibody was then incubated with the cells for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.
This image is courtesy of an Anonymous Abreview submitted on 12 April 2006.
All lanes : Anti-alpha Tubulin antibody [B-5-1-2] (ab11304) at 1/2000 dilution
Lane 1 : Whole cell lysate prepared from HEK293 cells
Lane 2 : Whole cell lysate prepared from HEK293 cells
Lane 3 : Whole cell lysate prepared from HEK293 cells
Lane 4 : Whole cell lysate prepared from HEK293 cells
Lane 5 : Whole cell lysate prepared from HEK293 cells
Lane 6 : Whole cell lysate prepared from HEK293 cells
Lysates/proteins at 30 µg per lane.
Secondary
HRP conjugated mouse anti-rabbit at 1/5000 dilution
developed using the ECL technique
Predicted band size : 50 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
Image courtesy of an anonymous Abreview.
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