Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)

Dear Sir/Madam,

I am a user of the antibody ab11304 and would appreciate if you could pass on to me precise details about the epitope used to generate that specific antibody.

Thank you for your help.

ANSWER:

Thank you for contacting us.

I can confirm that the immungen used to generate this anti-alpha Tubulin antibody clone [B-5-1-2] was a preparation of sarkosyl-resistant filaments from Stronglycentrotus purpuratus (sea urchin), as stated on the datasheet. Unfortunately, we do not have access to details about this preparation or an exact immunogen sequence for this particular antibody. However, the information we have suggests that the antibody recognises an epitope located at the C-terminal end of the alpha isoform of tubulin.

Several publications cite the use of this antibody clone, unfortunately we do not know whether any of these contain information on mapping of the epitope as we do not have access to the all of the articles themselves.

Preyer M et al. Interplay between Kinase Domain Autophosphorylation and F-Actin Binding Domain in Regulating Imatinib Sensitivity and Nuclear Import of BCR-ABL. PLoS One 6:e17020 (2011). PubMed: 21347248 Bou Kheir T et al. miR-449 inhibits cell proliferation and is down-regulated in gastric cancer. Mol Cancer 10:29 (2011). PubMed: 21418558 Gregersen LH et al. MicroRNA-145 targets YES and STAT1 in colon cancer cells. PLoS One 5:e8836 (2010). WB; Human. PubMed: 20098684 Sun Y et al. Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal myogenesis. J Cell Biol 189:1157-69 (2010).PubMed: 20566686 Zhang C et al. Amino acid-sensing mTOR signaling is involved in modulation of lipolysis by chronic insulin treatment in adipocytes. Am J Physiol Endocrinol Metab 296:E862-8 (2009). PubMed: 19190264 Zhou J et al. mTOR supports long-term self-renewal and suppresses mesoderm and endoderm activities of human embryonic stem cells. Proc Natl Acad Sci U S A 106:7840-5 (2009). PubMed: 19416884 Barnett KR et al. The aryl hydrocarbon receptor affects mouse ovarian follicle growth via mechanisms involving estradiol regulation and responsiveness. Biol Reprod 76:1062-70 (2007). PubMed: 17329597 Gossye V et al. Differential mechanism of NF-kappaB inhibition by two glucocorticoid receptor modulators in rheumatoid arthritis synovial fibroblasts. Arthritis Rheum 60:3241-50 (2009). PubMed: 19877072 Shelton, G.D., et al., Myopathy with tubulin-reactive inclusions in two cats. Acta Neuropathologica, 114(5), 537-542 (2007). Zhuang, Z.-G., et al., Suberoyl bis-hydroxamic acid induces p53-dependent apoptosis of MCF-7 breast cancer cells. Acta Pharmacologica Sinica, 29(12), 1459–1466 (2008). Komeno, Y., et al., AID-induced T-lymphoma or B-leukemia/lymphoma in a mouse BMT model. Leukemia, 24, 1018-1024 (2010). Banz, V.M., et al., Hsp90 transcriptionally and post-translationally regulates the expression of NDRG1 and maintains the stability of its modifying kinase GSK3β. Biochim. Biophys Acta (BBA) - Molecular Cell Research, 1793(10), 1597-1603 (2009). Gong, X., et al., RanBPM is an acetylcholinesterase-interacting protein that translocates into the nucleus during apoptosis. Acta Biochim. Biophys. Sinica, 41(11), 883-891 (2009). Okun, E., et al., TLR2 activation inhibits embryonic neural progenitor cell proliferation. J. Neurochem., 114(2), 462–474 (2010).

We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. Should you have any further questions, please do not hesitate to contact us.

I am interesting in finding a mature neuronal marker (like tubulin or neurofilament) that is specific to mouse BUT does NOT cross react to gerbil. I am using these two animal models and I need to differentiate cells from these two sources. I would appreciate help in finding this specific antibody.

ANSWER:

Thank you for your email. The best way to search the neuronal markers that we carry is to do the following: under product browser (located on the left hand side of the Abcam homepage) click on Tags & Markers, then Cell Type Markers, then Neuroscience Markers, and then Neuronal. You will then be able to view all the neuronal markers that we have.

Species cross-reactivity for each antibody will be indicated on the right hand side on the list and for more specific information regarding the antibodies, please refer to the individual online datasheets. If a particular species has been tested, the information will located on the online datasheet.

If you have any further questions, please contact us again.