![Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [B-5-1-2] (ab11304) image](#ab11304_1.jpg)
![Western blot - alpha Tubulin antibody [B-5-1-2] (ab11304) image](#alpha-Tubulin-Primary-antibodies-ab11304-6.jpg)
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Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Overview
Product name
Anti-alpha Tubulin antibody [B-5-1-2]
See all alpha Tubulin products (26) ...
Description
Mouse monoclonal [B-5-1-2] to alpha Tubulin
Tested applications
WB, RIA, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Cow, Human, Chlamydomonas reinhardtii, African Green Monkey, Sea Urchin
Immunogen
Sarkosyl-resistant filaments from Stronglycentrotus purpuratus (sea urchin).
Epitope
The antibody recognizes an epitope located at the C-terminal end of the alpha tubulin isoform.
Positive control
Total cell extract of the human foreskin fibroblast cell line (FS11).
General notes
Storage in frost-free freezers is also not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
B-5-1-2
Isotype
IgG1
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.25 - 0.5 µg/ml.Predicted molecular weight: 50 kDa.
RIA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
ICC/IF: 1/100
Target
Relevance
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
Cellular localization
Cytoplasmic
Alternative names
- Alpha tubulin 2 antibody
- Alpha tubulin 3 antibody
- Alpha tubulin 3C/D antibody
see all
Database links
- Entrez Gene: 404053 Cow
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 7846 Human
- Entrez Gene: 22142 Mouse
- Entrez Gene: 64158 Rat
- Omim: 191110 Human
- Omim: 602528 Human
see all
Anti-alpha Tubulin antibody [B-5-1-2] images:
Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [B-5-1-2] (ab11304)
![Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [B-5-1-2] (ab11304)](/ps/datasheet/Images/11/ab11304/ab11304_1.jpg)
ab11304 at a 1/100 dilution staining human HeLa cells by Immunocytochemistry/Immunofluorescence. The cells were methanol fixed and blocked for 1 hour with 10% serum. The antibody was then incubated with the cells for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.
This image is courtesy of an Anonymous Abreview submitted on 12 April 2006.
Western blot - alpha Tubulin antibody [B-5-1-2] (ab11304)
![Western blot - alpha Tubulin antibody [B-5-1-2] (ab11304)](/ps/datasheet/images/11/ab11304/alpha-Tubulin-Primary-antibodies-ab11304-6.jpg)
All lanes : Anti-alpha Tubulin antibody [B-5-1-2] (ab11304) at 1/2000 dilution
Lane 1 : Whole cell lysate prepared from HEK293 cells
Lane 2 : Whole cell lysate prepared from HEK293 cells
Lane 3 : Whole cell lysate prepared from HEK293 cells
Lane 4 : Whole cell lysate prepared from HEK293 cells
Lane 5 : Whole cell lysate prepared from HEK293 cells
Lane 6 : Whole cell lysate prepared from HEK293 cells
Lysates/proteins at 30 µg per lane.
Secondary
HRP conjugated mouse anti-rabbit at 1/5000 dilution
developed using the ECL technique
Predicted band size : 50 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
Image courtesy of an anonymous Abreview.
References for Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)
This product has been referenced in:
- Preyer Met al. Interplay between Kinase Domain Autophosphorylation and F-Actin Binding Domain in Regulating Imatinib Sensitivity and Nuclear Import of BCR-ABL. PLoS One 6:e17020 (2011). WB; African Green Monkey.Read more (PubMed: 21347248) »
- Bou Kheir Tet al. miR-449 inhibits cell proliferation and is down-regulated in gastric cancer. Mol Cancer 10:29 (2011). WB; Mouse.Read more (PubMed: 21418558) »
See all 8 publications for this product
Publishing research using ab11304? Please let us know so that we can cite the reference in this datasheet
![alpha Tubulin antibody [B-5-1-2] for Western blot in Mouse (11304)](/ps/datasheet/images/11/ab11304/alpha-Tubulin-Primary-antibodies-ab11304-5.jpg)
![alpha Tubulin antibody [B-5-1-2] for Western blot in Human (11304)](/ps/datasheet/images/11/ab11304/alpha-Tubulin-Primary-antibodies-ab11304-4.jpg)
![alpha Tubulin antibody [B-5-1-2] for Western blot in Mouse (11304)](/ps/datasheet/images/11/ab11304/alpha-Tubulin-Primary-antibodies-ab11304-1.jpg)
![alpha Tubulin antibody [B-5-1-2] for ICC/IF in Human (11304)](/ps/Reviews/Images/ab4521_13913.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"