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Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [B-5-1-2] (ab11304)
![Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [B-5-1-2] (ab11304)](/ps/datasheet/images/ab11304_1.jpg)
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Product Name
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alpha Tubulin antibody [B-5-1-2]
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See all Tubulin (alpha) antibodies (20)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [B-5-1-2] to alpha Tubulin
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Immunogen
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Sarkosyl-resistant filaments from Stronglycentrotus purpuratus (sea urchin).
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Reacts with
(species key)
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Hu, Ms, Rat, AGMk, Chk, C rei, Cow, S urc
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Epitope
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The antibody recognizes an epitope located at the C-terminal end of the alpha tubulin isoform.
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Tested applications
(see key)
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ICC/IF, IP, RIA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at a concentration of 0.5 - 1 µg/ml.
ICC/IF: Used at a dilution of 1/100 for 1 hr on HeLa cells (see Abreview).
IP: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: Use at a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 50 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Total cell extract of the human foreskin fibroblast cell line (FS11).
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
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Relevance
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Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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B-5-1-2
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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2.300 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Storage in frost-free freezers is also not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this alpha Tubulin antibody [B-5-1-2] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for alpha Tubulin antibody [B-5-1-2] images, references, products related to ab11304 and other tools.
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alpha Tubulin antibody [B-5-1-2] images:
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 Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [B-5-1-2] (ab11304)
ab11304 at a 1/100 dilution staining human HeLa cells by Immunocytochemistry/Immunofluorescence. The cells were methanol fixed and blocked for 1 hour with 10% serum. The antibody was then incubated with the cells for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody. This image is courtesy of an Anonymous Abreview submitted on 12 April 2006.
See Abreview
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References for alpha Tubulin antibody [B-5-1-2] (ab11304)
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This product has been used in: (two most recent references)
Gregersen LH et al. MicroRNA-145 targets YES and STAT1 in colon cancer cells. PLoS One 5:e8836 (2010). WB; Human. PubMed: 20098684
Zhang C et al. Amino acid-sensing mTOR signaling is involved in modulation of lipolysis by chronic insulin treatment in adipocytes. Am J Physiol Endocrinol Metab 296:E862-8 (2009). WB; Mouse. PubMed: 19190264
See all 4 publication references for this product.
If you publish research using ab11304 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to alpha Tubulin
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alpha Tubulin antibody [B-5-1-2] - more information
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Customer reviews (feedback) regarding alpha Tubulin antibody [B-5-1-2] |
Customer FAQs regarding alpha Tubulin antibody [B-5-1-2] |
Protocols for alpha Tubulin antibody [B-5-1-2] |
Price and availability of products related to alpha Tubulin antibody [B-5-1-2] |
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alpha Tubulin antibody [B-5-1-2] - related products:
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alpha Tubulin antibody [B-5-1-2] - other tools:
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Contact Abcam with a Technical Enquiry about alpha Tubulin antibody [B-5-1-2] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [B-5-1-2] (ab11304)](/index.html?datasheet=11304#ab11304_1.jpg)
