Anti-alpha Tubulin antibody (Biotin) (ab106042)
- Product nameAnti-alpha Tubulin antibody (Biotin)See all alpha Tubulin primary antibodies ...
- DescriptionRabbit polyclonal to alpha Tubulin (Biotin)
- SpecificityCross reactivity will occur with all isoforms of alpha tubulin.
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
Synthetic peptide: ALEKEGEGEEEGEEY, corresponding to amino acids 427-441 of Human alpha Tubulin
- Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, 1% BSA
BSA is Immunoglobulin and Protease free
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesab106042 was affinity purified from monospecific antiserum by immunoaffinity purification.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab106042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/2000 - 1/10000. Predicted molecular weight: 50 kDa.|
|ELISA||ELISA: 1/20000 - 1/100000.|
- FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
- Sequence similaritiesBelongs to the tubulin family.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
- Cellular localizationCytoplasm > cytoskeleton.
- Alpha-tubulin 1 antibody
- B ALPHA 1 antibody
- bA408E5.3 antibody
- H2 ALPHA antibody
- Hum a tub1 antibody
- Hum a tub2 antibody
- LIS3 antibody
- TBA4A_HUMAN antibody
- Testis-specific alpha-tubulin antibody
- TUBA1 antibody
- TUBA1A antibody
- TUBA2 antibody
- TUBA3 antibody
- TUBA3C antibody
- TUBA4A antibody
- Tubulin alpha 1 chain antibody
- Tubulin alpha antibody
- Tubulin alpha-1 chain antibody
- Tubulin alpha-4A chain antibody
- Tubulin H2-alpha antibody
- Tubulin, alpha 1 (testis specific) antibody
- Tubulin, alpha 1a antibody
- Tubulin, alpha 3c antibody
- Tubulin, alpha 4a antibody
- Tubulin, alpha, testis-specific antibody
- Tubulin, alpha-1 antibody
References for Anti-alpha Tubulin antibody (Biotin) (ab106042)
ab106042 has not yet been referenced specifically in any publications.