Anti-alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker (ab64503)

Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [DM1A] (FITC) (ab64503)

ICC/IF image of ab64503 stained human HeLa cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab64503, 1µg/ml, FITC conjugated (green)) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Immunohistochemistry (Frozen sections) - alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker (ab64503)

ab64503 staining alpha Tubulin in Xenopus laevis stage 36, transverse cryosection tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with MEMFa and blocked with 2% BSA/Serum for 1 hour at 23⁰C. The sample was incubated with primary antibody (1/100) for 1 hour at 23⁰C. No secondary antibody was used. FITC-tubulin shown in green highlights neurons in neural tube and ciliated epidermal cells. DAPI shows staining in grey. Laminin (Abcam`s ab11575) is in blue (donkey anti-rabbit Cy5 secondary). E-cadherin is in red (donkey anti-mouse Cy3 secondary).

This image is a courtesy of Abreview submitted by Anonymous

Flow Cytometry-Anti-alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker(ab64503)

Overlay histogram showing HeLa cells stained with ab64503 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64503, 0.5µg/1x10⁶ cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x10⁶ cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.