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Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [DM1A] (FITC) (ab64503)
![Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [DM1A] (FITC) (ab64503)](/ps/datasheet/images/ab7291-IF-Im4.jpg)
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Immunohistochemistry (Frozen sections) - alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker (ab64503)
![Immunohistochemistry (Frozen sections) - alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker (ab64503)](/ps/datasheet/images/64/ab64503/alpha-Tubulin-Primary-antibodies-ab64503-4.jpg)
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Product Name
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alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker
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See all alpha Tubulin - Loading Control antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (FITC)
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Immunogen
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Full length native protein (purified) of Chicken alpha Tubulin (extracted from brain).
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Reacts with
(species key)
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Hu, Ms, Rat, Chk, Cow, Dog, Grb, Gpig, Pig, Xl
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Specificity
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Alpha Tubulin clone DM1A causes the 10 nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. It does not inhibit polymerisation or depolymerisation of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. See references for further information on the above.
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Epitope
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aa426-450.
Ab64503 specifically recognizes an epitope in the carboxy-terminal part of alpha-tubulin.
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Tested applications
(see key)
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ICC/IF, IHC-Fr
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml. Unconjugated version available as ab7291.
IHC-Fr: 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cells.
Cultured human fibroblasts, baby hamster kidney (BHK) cells.
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Cellular localization
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Major constituent of Microtubules
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Isotype/Loading Controls >> Loading Controls >> Tubulin
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Relevance
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Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Alpha tubulin is not suitable as a loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1): 53-60, "in cells undergoing adipose differentiation actin synthesis decreases by 90%").
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Alternative names alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker (ab64503) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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DM1A
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Conjugation
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Fluorescein
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C in the dark. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker images, references, products related to ab64503 and other tools.
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alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker images:
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 Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [DM1A] (FITC) (ab64503)
ICC/IF image of ab64503 stained human HeLa cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab64503, 1µg/ml, FITC conjugated (green)) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Immunohistochemistry (Frozen sections) - alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker (ab64503)
ab64503 staining alpha Tubulin in Xenopus laevis stage 36, transverse cryosection tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with MEMFa and blocked with 2% BSA/Serum for 1 hour at 230C. The sample was incubated with primary antibody (1/100) for 1 hour at 230C. No secondary antibody was used. FITC-tubulin shown in green highlights neurons in neural tube and ciliated epidermal cells. DAPI shows staining in grey. Laminin (ab11575) is in blue (donkey anti-rabbit Cy5 secondary). E-cadherin is in red (donkey anti-mouse Cy3 secondary). This image is a courtesy of Abreview submitted by Anonimous
See Abreview
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References for alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker (ab64503)
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This product has been used in: (two most recent references)
Wray J et al. Metnase mediates chromosome decatenation in acute leukemia cells. Blood 114:1852-8 (2009). ICC/IF; Human. PubMed: 19458360
Habela CW et al. ClC3 is a critical regulator of the cell cycle in normal and malignant glial cells. J Neurosci 28:9205-17 (2008). PubMed: 18784301
See all 3 publication references for this product.
If you publish research using ab64503 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to alpha Tubulin
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alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker - more information
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Protocols for alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker |
Price and availability of products related to alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker |
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alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker - related products:
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alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker - other tools:
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Contact Abcam with a Technical Enquiry about alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [DM1A] (FITC) (ab64503)](/index.html?datasheet=64503#ab7291-IF-Im4.jpg)
![Immunohistochemistry (Frozen sections) - alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker (ab64503)](/index.html?datasheet=64503#alpha-Tubulin-Primary-antibodies-ab64503-4.jpg)
