Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Isotype/Loading Controls >> Loading Controls >> Tubulin
Overview
Product name
Anti-alpha Tubulin antibody - Loading Control
See all alpha Tubulin products (5) ...
Description
Rabbit polyclonal to alpha Tubulin - Loading Control
Specificity
This antibody detects a single band in cell lines, corresponding to alpha tubulin.
Tested applications
IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Cow, Human
Predicted to work with
Pig, Xenopus laevis, Chimpanzee, Monkey, Zebrafish, Chinese Hamster
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human alpha Tubulin.
(Peptide available as ab23537.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
This antibody makes an excellent loading control.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Isotype/Loading Controls >> Loading Controls >> Tubulin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/25.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Can be blocked with alpha Tubulin peptide (ab23537).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Alpha tubulin is not suitable as a loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1): 53-60, "in cells undergoing adipose differentiation actin synthesis decreases by 90%").
Cellular localization
Major constituent of Microtubules
Alternative names
- Alpha-tubulin 1 antibody
- FLJ30169 antibody
- H2 ALPHA antibody
see all
Database links
- Entrez Gene: 429035 Chicken
- Entrez Gene: 504244 Cow
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 7846 Human
- Entrez Gene: 22142 Mouse
- Entrez Gene: 64158 Rat
- Entrez Gene: 399313 Xenopus laevis
see all
Anti-alpha Tubulin antibody - Loading Control images:
Western blot - alpha Tubulin antibody - Loading Control (ab4074)
All lanes : Anti-alpha Tubulin antibody - Loading Control (ab4074) at 1 µg/ml
Lane 1 : Mouse 3T3 lysate
Lane 2 : Rat liver lysate
Lane 3 : Cow cell lysate
Lane 4 : Chicken cell lysate
Lane 5 : CHO lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 50 kDa
Exposure time : 30 seconds
ab4074 cross-reacts strongly with Mouse 3T3 lysate and shows weak cross-reactivity with Rat liver, Cow, Chicken and CHO lysates.
Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody - Loading Control (ab4074)
ICC/IF image of ab4074 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab4074, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
Western blot - alpha Tubulin antibody - Loading Control (ab4074)
All lanes : Anti-alpha Tubulin antibody - Loading Control (ab4074) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate with alpha Tubulin peptide (ab23537) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
Predicted band size : 50 kDa
Observed band size : 50 kDa
Immunohistochemistry (Paraffin-embedded sections) - alpha Tubulin antibody - Loading Control (ab4074)
(Image courtesy of Human Protein Atlas).
ab4074 staining alpha tubulin in the glandular cells of the endometrium. Paraffin embedded endometrium tissue of a 33 year-old female was incubated with ab4074 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab4074 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
.Western blot - alpha Tubulin antibody - Loading Control (ab4074)
Anti-alpha Tubulin antibody - Loading Control (ab4074) at 1 µg/ml + whole cell lysate prepared from MCF7 cells at 20 µg
Secondary
Goat anti-rabbit IgG-HRP at 1/1000 dilution
developed using the ECL technique
Predicted band size : 50 kDa
Observed band size : 50 kDa
Exposure time : 5 minutes
Image courtesy of an anonymous Abreview.
References for Anti-alpha Tubulin antibody - Loading Control (ab4074)
This product has been referenced in:
- Raiz Jet al. The non-autonomous retrotransposon SVA is trans-mobilized by the human LINE-1 protein machinery. Nucleic Acids Res : (2011). WB; Human.Read more (PubMed: 22053090) »
- He Yet al. STAMP alters the growth of transformed and ovarian cancer cells. BMC Cancer 10:128 (2010). WB; Human.Read more (PubMed: 20374646) »
See all 14 publications for this product
Publishing research using ab4074? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"