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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human alpha Tubulin.
(Peptide available as ab23537.)
This antibody makes an excellent loading control.
Our Abpromise guarantee covers the use of ab4074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human alpha Tubulin peptide (ab23537). Can be blocked with alpha Tubulin peptide (ab23537).|
|Flow Cyt||1/800. Ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 1 µg/ml.|
ab4074 cross-reacts strongly with Mouse 3T3 lysate and shows weak cross-reactivity with Rat liver, Cow, Chicken and CHO lysates.
Image courtesy of an anonymous Abreview.
ab4074 staining Tubulin in human LGE neural progenitors by Flow Cytometry. Cells were paraformaldehyde and permeabilized. The sample was incubated with the primary antibody (1/500) for 1 hour at 20°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/800) was used as the secondary antibody.
Gating Strategy: Isotype population (shown in white).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"