Anti-alpha Tubulin antibody [SPM359], prediluted (ab54446)

Overview

  • Product nameAnti-alpha Tubulin antibody [SPM359], predilutedSee all alpha Tubulin primary antibodies ...
  • Description
    Mouse monoclonal [SPM359] to alpha Tubulin, prediluted
  • Tested applicationsIHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Gerbil
  • Immunogen

    Native chick brain microtubules.

  • Epitopeab54446 reacts with an epitope located in the region encoded by amino acids 426-450.
  • Positive control
    • Ls174T, MAD109 cells. Skin or lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab54446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: Ready-to-use for 30 min at RT.
    No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • Sequence similaritiesBelongs to the tubulin family.
    • Post-translational
      modifications
      Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
      Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha tubulin ubiquitous antibody
      • Alpha-tubulin 1 antibody
      • B ALPHA 1 antibody
      • bA408E5.3 antibody
      • H2 ALPHA antibody
      • Hum a tub1 antibody
      • Hum a tub2 antibody
      • LIS3 antibody
      • TBA4A_HUMAN antibody
      • Testis-specific alpha-tubulin antibody
      • TUBA1 antibody
      • TUBA1A antibody
      • Tuba4a antibody
      • Tubulin alpha 1 chain antibody
      • Tubulin alpha antibody
      • Tubulin alpha ubiquitous chain antibody
      • Tubulin alpha-1 chain antibody
      • Tubulin alpha-4A chain antibody
      • Tubulin H2-alpha antibody
      • Tubulin K alpha 1 antibody
      • Tubulin, alpha 1 (testis specific) antibody
      • Tubulin, alpha 4a antibody
      • Tubulin, alpha, testis-specific antibody
      • Tubulin, alpha-1 antibody
      see all

    Anti-alpha Tubulin antibody [SPM359], prediluted images

    • Ab54446 staining human alpha Tubulin in human lung tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    References for Anti-alpha Tubulin antibody [SPM359], prediluted (ab54446)

    ab54446 has not yet been referenced specifically in any publications.

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