Anti-alpha Tubulin antibody [TU-01] (Biotin) (ab74696)

Overview

  • Product nameAnti-alpha Tubulin antibody [TU-01] (Biotin)See all alpha Tubulin primary antibodies ...
  • Description
    Mouse monoclonal [TU-01] to alpha Tubulin (Biotin)
  • ConjugationBiotin
  • Conjugation notesThe purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
  • Specificityab74696 recognizes the defined epitope (aa 65-97) on the N-terminal structural domain of alpha-tubulin. This epitope is conserved within all species.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.

Properties

Applications

Our Abpromise guarantee covers the use of ab74696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Predicted molecular weight: 54 kDa.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Alpha tubulin ubiquitous antibody
    • Alpha-tubulin 1 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha ubiquitous chain antibody
    • Tubulin alpha-1 chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin K alpha 1 antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

References for Anti-alpha Tubulin antibody [TU-01] (Biotin) (ab74696)

ab74696 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and your interest in our products. Anti-alpha tubulin (clone TU-01) can be used as loading control together with antibody HLA Class I (ab23755) . Currently, we have several forms of this antibody ie. unconjugated as well as c...

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I think my first guess when we spoke was 3-6 biotins per IgG, but the lab says 4-8. I hope this helps. If you are trying to compare the relative signal you can expect from different anti-tubulin antibodies, this followed by an avidin or streptavidn con...

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