Anti-alpha Tubulin antibody [TU-01] (FITC) (ab18247)
- Product nameAnti-alpha Tubulin antibody [TU-01] (FITC)See all alpha Tubulin primary antibodies ...
- DescriptionMouse monoclonal [TU-01] to alpha Tubulin (FITC)
- ConjugationFITC. Ex: 493nm, Em: 528nm
- Tested applicationsICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human, Pig
Fraction of tubulin purified from pig brain by two cycles of polymerization-depolymerization.
- Epitopeab18247 recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha tubulin.
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
- Concentration information loading...
- PurityIgG fraction
- Purification notesab18247 purified from pig brain by two cycles of polymerization-depolymerization. Purified ab18247 is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
- Clonality Monoclonal
- Clone numberTU-01
- Research Areas
Our Abpromise guarantee covers the use of ab18247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
- Sequence similaritiesBelongs to the tubulin family.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
- Cellular localizationCytoplasm > cytoskeleton.
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- B ALPHA 1 antibody
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- H2 ALPHA antibody
- Hum a tub1 antibody
- Hum a tub2 antibody
- LIS3 antibody
- TBA4A_HUMAN antibody
- Testis-specific alpha-tubulin antibody
- TUBA1 antibody
- TUBA1A antibody
- Tuba4a antibody
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- Tubulin alpha ubiquitous chain antibody
- Tubulin alpha-1 chain antibody
- Tubulin alpha-4A chain antibody
- Tubulin H2-alpha antibody
- Tubulin K alpha 1 antibody
- Tubulin, alpha 1 (testis specific) antibody
- Tubulin, alpha 4a antibody
- Tubulin, alpha, testis-specific antibody
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References for Anti-alpha Tubulin antibody [TU-01] (FITC) (ab18247)
ab18247 has not yet been referenced specifically in any publications.