Anti-alpha Tubulin antibody [TU-01] (ab7750)

Western blot - alpha Tubulin antibody [TU-01] (ab7750)

All lanes : Anti-alpha Tubulin antibody [TU-01] (ab7750) at 1/500 dilution

Lane 1 : Dog 1 heart whole tissue lysate (left ventricle)
Lane 2 : Dog 2 heart whole tissue lysate(left ventricle)
Lane 3 : Dog 3 heart whole tissue lysate (left ventricle)
Lane 4 : Dog 4 heart whole tissue lysate (left ventricle)

Lysates/proteins at 80 µg per lane.

Secondary
HRP conjugated sheep polyclonal antibody
developed using the ECL technique

Performed under reducing conditions.

Observed band size : 55 kDa (why is the actual band size different from the predicted?)


Exposure time : 20 minutes

This image is courtesy of an Abreview submitted by Dr sudhish mishra

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-alpha Tubulin antibody [TU-01](ab7750)

Ab7750 staining human normal skin. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Flow Cytometry-alpha Tubulin antibody [TU-01](ab7750)

Overlay histogram showing HEK293 cells stained with ab7750 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton® X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7750, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton® X-100 used under the same conditions.