Anti-alpha Tubulin antibody [TU-01] (ab7750)

To whom it may concern, Do you have any loading controls that would be suitable to use in a Western blot with any of your anti-HLA class 1 antibodies such as ab23755? Thanks and best wishes,

ANSWER:

Thank you for your enquiry and your interest in our products. Anti-alpha tubulin (clone TU-01) can be used as loading control together with antibody HLA Class I (ab23755) . Currently, we have several forms of this antibody ie. unconjugated as well as conjugated: - ab7750 - Anti-alpha Tubulin antibody [TU-01] - ab18247 - Anti-alpha Tubulin antibody [TU-01] (FITC) - ab46708 - Anti-alpha Tubulin antibody [TU-01] (Dyomics® 547) - ab74696 - Anti-alpha Tubulin antibody [TU-01] (Biotin) - ab78108 - Anti-alpha Tubulin antibody [TU-01] I hope this helps and if I can assist further, please do not hesitate to contact me.

Dear Technical Team,   Hope this e-mail finds you well!   One of our customer is working on the separation of the nuclear and cytoplasmic fractions from mammalian cells like HEK 293 (HUMAN) and PS Cells(Porcine-Pig) & wants to make sure that there is no contamination of nuclear fraction in cytoplasmic fraction and vice versa. For that she wants to probe nucleus and cytoplam specific proteins in western blotting.   Kindly advise the suitable antibodies which can detect the cytoplasmic proteins & not the nuclear.   Looking forward towards your valued response on it.   With Best Regards,  

ANSWER:

Thank you for your enquiry.

I can confirm that the loading control protein alpha tubulin should only be located in the cytoplasm. We have several antibodies for detection of alpha tubulin which have been tested in WB, human and pig. I can recommend to review the datasheets for further information.

ab15246 Anti-alpha Tubulin antibody Rabbit polyclonal Applications: ICC/IF, IHC-Fr, IHC-P, IP, WB Reacts with: Fruit fly (Drosophila melanogaster), Human, Mouse, Pig, Rat, Zebrafish Click here (or use the following: http://www.abcam.com/index.html?datasheet=15246).

ab7750 Anti-alpha Tubulin antibody [TU-01] Mouse monoclonal Applications: ELISA, Flow Cyt, ICC, IF, IHC-P, IP, WB Reacts with: Human, Mouse, Arabidopsis thaliana, Cow, Dog, Pig Click here (or use the following: http://www.abcam.com/index.html?datasheet=7750). ab80779 Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free Mouse monoclonal Applications: EM, Flow Cyt, ICC/IF, IHC-P, IP, WB Reacts with: Human, Mouse, Rat, Chicken, Cow, Gerbil, Guinea pig, Pig, Xenopus laevis Click here (or use the following: http://www.abcam.com/index.html?datasheet=80779).

If you require any further assistance to find a suitable nuclear marker, please do not hesitate to let me know.

I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

Sample species is Fungus. He works on immuno staining. Stain with Goat Anti-Mouse IgG (γ-chain specific) FITC antibody.

From our product, we cannot find for fungus, buy #ab7750 is "many" Please let us know which alpha tubulin you will recommended to work.

ANSWER:

I am sorry for the delayed response. We have not tested it in Fungi but we would expect it to react with Fungi. The secondary goat anti-mouse is compatible with ab7750. I hope this helps. Please do not hesitate to contact us if you have any further questions.

Hi tech dept., We are interested in purchasing ab7750 as a loading control for western blotting. We were wondering if this antibody is raised against all the alpha tubulin isoforms? The other enquiry is that recently we have changed our detection system and how use a product by Peirce (USA) called west dura with a CCD camera, we are finding this much better than traditional film. However this type of detection system does not allow sodium azide to be added as a preservative to antibodies, therefore is it possible to supply antibodies without this preservative?

Many thanks

ANSWER:

I have received more information about ab7750:

The antibody was raised against pig brain microtubeule preparation. The epitope was mapped within 37-154 aa sequence (N-terminal domain). It reacts with wide variety of species including eukaryotes, however not completely all species (some moulds and yeast were negative).

Unfortunately the more detailed characterization concerning individual alpha tubulin isoforms reactivity has not been performed.

Please do not hesitate to contact us if you need any more advice or information,

I want to buy alpha-tubulin antibody(ab7750,TU01)from you. But I amnot sure if this antibody could be used in IP experiment. Could you recommendate me some published papers, in which ab7750,TU01 was used in IP EXPERIMENT? What the dilute ratio of ab7750 was used in IP EXPERIMENT? Could you please tell me how could I order it from your company in USA? Thanks!!

ANSWER:

According to the datasheet, this antibody has been tested for IP application. We have five published papers on this product, however we do not know which one is about IP application. I would suggest that you should carry out a preliminary experiment with different dilutions (i.e.1:100, 1:500 and 1:1000) with a fixed amount of antigen (10-100 ng target protein) and then adjust the dilution accordingly to your results obtained.