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Anti-alpha Tubulin antibody [TU-01] (ab78108)

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Overview

  • Product nameAnti-alpha Tubulin antibody [TU-01]See all alpha Tubulin primary antibodies ...
  • Description
    Mouse monoclonal [TU-01] to alpha Tubulin
  • SpecificityRecognizes all alpha Tubulin isoforms.
  • Tested applicationsWB, IHC-P, ICC more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Fraction of tubulin purified from porcine brain by two cycles of polymerization-depolymerization

  • EpitopeRecognises a defined epitope (aa 65-97) on the N-terminal structural domain of alpha Tubulin.
  • Positive controlHeart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab78108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P IHC-P: Use a concentration of 5 µg/ml.
ICC ICC: Use at an assay dependent dilution.

Target

  • Alternative names
      Alpha tubulin 2 antibodyAlpha tubulin 3 antibodyAlpha tubulin 3C/D antibody
      B ALPHA 1 antibodybA408E5.3 antibodyH2 ALPHA antibodyHum a tub1 antibodyHum a tub2 antibodyLIS3 antibodyTUBA1 antibodyTUBA1A antibodyTUBA2 antibodyTUBA3 antibodyTUBA3C antibodyTUBA3D antibodyTUBA4A antibodyTubulin alpha 1 chain antibodyTubulin alpha 1A chain antibodyTubulin alpha 2 chain antibodyTubulin alpha 3 chain antibodyTubulin alpha 3C/D chain antibodyTubulin alpha antibodyTubulin B alpha 1 antibodyTubulin K alpha 1 antibodyTubulin, alpha 1 (testis specific) antibodyTubulin, alpha 1a antibodyTubulin, alpha 2 antibodyTubulin, alpha 3 antibodyTubulin, alpha 3c antibodyTubulin, alpha 4a antibodyTubulin, alpha, brain specific antibody
    see all

Anti-alpha Tubulin antibody [TU-01] images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Tubulin antibody [TU-01] (ab78108)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Tubulin antibody [TU-01] (ab78108)
    Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human heart tissue stained with 5µg/ml ab78108.

    A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-alpha Tubulin antibody [TU-01] (ab78108)

ab78108 has not yet been referenced specifically in any publications.

Publishing research using ab78108 ? Please let us know so that we can cite the reference in this datasheet

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To whom it may concern, Do you have any loading controls that would be suitable to use in a Western blot with any of your anti-HLA class 1 antibodies such as ab23755? Thanks and best wishes,

Thank you for your enquiry and your interest in our products. Anti-alpha tubulin (clone TU-01) can be used as loading control together with antibody HLA Class I (ab23755) . Currently, we have several forms of this antibody ie. unconjugated as well as conj...

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Dear Technical Support, I was wondering whether your anti-α tubulin antibody (AB78108) will work on protein extracts from rat tissues (for Western Blot). I noticed on your website that cross reactivity is predicted in a wide range of species but is this...

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Thank you very much for your interest in ab78108. To our knowledge, ab78108 has not been tested specifically  in Rat  (only in human). However, it is  predicted to react with a wide range of other species including rat due to sequnce homology.  Theref...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"