Anti-alpha Tubulin antibody [TU-16] (ab7749)

Overview

• Product nameAnti-alpha Tubulin antibody [TU-16]See all alpha Tubulin primary antibodies ...
• Description
  Mouse monoclonal [TU-16] to alpha Tubulin
• SpecificityThe antibody binds tubulin under denaturing and non-denaturing conditions.
• Tested applicationsIHC-Fr, IHC-P, ICC, IP, WB more details
• Species reactivity
  Reacts with: Chicken, Pig
  Predicted to work with: Mouse, Rat, Hamster, Cow, Human, Plants
• Immunogen

  Full length native protein (purified) (Pig).

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• Purity>95% by SDS-PAGE
• Purification notesPurified from ascites using precipitation methods.
• Clonality Monoclonal
• Clone numberTU-16
• IsotypeIgM
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microtubules
  • Tubulin

  • Tags & Cell Markers
  • Subcellular Markers
  • Cytoskeleton
  • Microtubules

Applications

Target

• RelevanceTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
• Cellular localizationCytoplasmic
• Database links
  • Entrez Gene: 404053 Cow
  • Entrez Gene: 7277 Human
  • Entrez Gene: 7278 Human
  • Entrez Gene: 7846 Human
  • Entrez Gene: 22142 Mouse
  • Entrez Gene: 100158003 Pig
  • Entrez Gene: 64158 Rat
  • Omim: 191110 Human

  • Omim: 602529 Human
  • Omim: 602528 Human
  • SwissProt: P02552 Chicken
  • SwissProt: P81947 Cow
  • SwissProt: P68366 Human
  • SwissProt: Q71U36 Human
  • SwissProt: Q13748 Human
  • SwissProt: P68369 Mouse
  • SwissProt: Q5XJF8 Mouse
  • SwissProt: P02550 Pig
  • SwissProt: P68370 Rat

  see all

• Alternative names
  Alpha tubulin 2 antibodyAlpha tubulin 3 antibodyAlpha tubulin 3C/D antibody

  Alpha tubulin ubiquitous antibodyB ALPHA 1 antibodybA408E5.3 antibodyH2 ALPHA antibodyHum a tub1 antibodyHum a tub2 antibodyLIS3 antibodyTUBA1 antibodyTUBA1A antibodyTUBA2 antibodyTUBA3 antibodyTUBA3C antibodyTUBA3D antibodyTUBA4A antibodyTubulin alpha 1 chain antibodyTubulin alpha 1A chain antibodyTubulin alpha 2 chain antibodyTubulin alpha 3 chain antibodyTubulin alpha 3C/D chain antibodyTubulin alpha antibodyTubulin alpha ubiquitous chain antibodyTubulin B alpha 1 antibodyTubulin K alpha 1 antibodyTubulin, alpha 1 (testis specific) antibodyTubulin, alpha 1a antibodyTubulin, alpha 2 antibodyTubulin, alpha 3 antibodyTubulin, alpha 3c antibodyTubulin, alpha 4a antibodyTubulin, alpha, brain specific antibody

  see all

Anti-alpha Tubulin antibody [TU-16] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Tubulin antibody [TU-16] (ab7749)
  Ab7749 staining human normal skin. Staining is localised to the cytoplasm.
  Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
• 

  Immunohistochemistry (Frozen sections) - alpha Tubulin antibody [TU-16] (ab7749)Image from Ahlstrom JD et.al., Development 136, 1801-1812 (2009), Fig 7B.

  ab7749 at 1/500 dilution staining alpha tubulin in chicken embryo trunk section by Immunohistochemistry (Frozen sections). Embryonic trunks were electroporated at Hamburger and Hamilton (HH) stages 14-16 with mem-EGFP, harvested 16-19 hours after electroporation at HH stages 17-19 and cryosectioned at 30 μm. Cryosections were rehydrated in PBS, the cells permeabilized in -20°C acetone for 20 minutes, washed in PBS and then blocked in PBS containing 2% normal goat serum (1% BSA, 0.1% cold water fish skin gelatin, 0.1% Triton X-100 and 0.05% Tween 20) for 20 minutes. Blocking solution was removed and cryosections were incubated overnight with primary antibody at 4°C. A Cy5 conjugated goat anti mouse IgM at 1/300 dilution was used as secondary.