Overview

  • Product nameAnti-alpha Tubulin antibody [TU-16]See all alpha Tubulin primary antibodies ...
  • Description
    Mouse monoclonal [TU-16] to alpha Tubulin
  • SpecificityThe antibody binds tubulin under denaturing and non-denaturing conditions.
  • Tested applicationsIHC-Fr, IHC-P, ICC, IP, WBmore details
  • Species reactivity
    Reacts with: Chicken, Pig
    Predicted to work with: Mouse, Rat, Hamster, Cow, Human, Plants
  • Immunogen

    Full length native protein (purified) (Pig).

Properties

Applications

Our Abpromise guarantee covers the use of ab7749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
IHC-P
ICC
IP
WB
  • Application notesICC: Use at an assay dependent dilution.
    IHC-Fr: 1/500 (PMID 19429784).
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6.0 before commencing with IHC staining protocol.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • Sequence similaritiesBelongs to the tubulin family.
    • Post-translational
      modifications
      Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
      Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha tubulin ubiquitous antibody
      • Alpha-tubulin 1 antibody
      • B ALPHA 1 antibody
      • bA408E5.3 antibody
      • H2 ALPHA antibody
      • Hum a tub1 antibody
      • Hum a tub2 antibody
      • LIS3 antibody
      • TBA4A_HUMAN antibody
      • Testis-specific alpha-tubulin antibody
      • TUBA1 antibody
      • TUBA1A antibody
      • Tuba4a antibody
      • Tubulin alpha 1 chain antibody
      • Tubulin alpha antibody
      • Tubulin alpha ubiquitous chain antibody
      • Tubulin alpha-1 chain antibody
      • Tubulin alpha-4A chain antibody
      • Tubulin H2-alpha antibody
      • Tubulin K alpha 1 antibody
      • Tubulin, alpha 1 (testis specific) antibody
      • Tubulin, alpha 4a antibody
      • Tubulin, alpha, testis-specific antibody
      • Tubulin, alpha-1 antibody
      see all

    Anti-alpha Tubulin antibody [TU-16] images

    • Ab7749 staining human normal skin. Staining is localised to the cytoplasm.
      Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • ab7749 at 1/500 dilution staining alpha tubulin in chicken embryo trunk section by Immunohistochemistry (Frozen sections). Embryonic trunks were electroporated at Hamburger and Hamilton (HH) stages 14-16 with mem-EGFP, harvested 16-19 hours after electroporation at HH stages 17-19 and cryosectioned at 30 μm. Cryosections were rehydrated in PBS, the cells permeabilized in -20°C acetone for 20 minutes, washed in PBS and then blocked in PBS containing 2% normal goat serum (1% BSA, 0.1% cold water fish skin gelatin, 0.1% Triton X-100 and 0.05% Tween 20) for 20 minutes. Blocking solution was removed and cryosections were incubated overnight with primary antibody at 4°C. A Cy5 conjugated goat anti mouse IgM at 1/300 dilution was used as secondary.

    References for Anti-alpha Tubulin antibody [TU-16] (ab7749)

    This product has been referenced in:
    • Ahlstrom JD & Erickson CA The neural crest epithelial-mesenchymal transition in 4D: a ;tail' of multiple non-obligatory cellular mechanisms. Development 136:1801-12 (2009). IHC-Fr ; Chicken . Read more (PubMed: 19429784) »
    • Klaiman G  et al. Targets of caspase-6 activity in human neurons and Alzheimer disease. Mol Cell Proteomics : (2008). WB . Read more (PubMed: 18487604) »

    See all 6 Publications for this product

    Product Wall

    Thank you for your enquiry. The sensitivity is higher when you use a primary and then a secondary conjugated with fluorescence as this method enables amplification of the signal. It is also possible to apply secondary anti-mouse Cy3 labelled Ab to v...

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    We used classical blocking solution for Western blots - 5% skimmed milk in PBS which can also be used for diluting the antibody (1:1000 for pig brain lysate, titre not tested in cell lines).

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"