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Full length native protein (purified) (Pig).
Our Abpromise guarantee covers the use of ab7749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab7749 at 1/500 dilution staining alpha tubulin in chicken embryo trunk section by Immunohistochemistry (Frozen sections). Embryonic trunks were electroporated at Hamburger and Hamilton (HH) stages 14-16 with mem-EGFP, harvested 16-19 hours after electroporation at HH stages 17-19 and cryosectioned at 30 μm. Cryosections were rehydrated in PBS, the cells permeabilized in -20°C acetone for 20 minutes, washed in PBS and then blocked in PBS containing 2% normal goat serum (1% BSA, 0.1% cold water fish skin gelatin, 0.1% Triton X-100 and 0.05% Tween 20) for 20 minutes. Blocking solution was removed and cryosections were incubated overnight with primary antibody at 4°C. A Cy5 conjugated goat anti mouse IgM at 1/300 dilution was used as secondary.
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