Anti-alpha Tubulin antibody [TU-16] (ab78110)
- Product nameAnti-alpha Tubulin antibody [TU-16]See all alpha Tubulin primary antibodies ...
- DescriptionMouse monoclonal [TU-16] to alpha Tubulin
- Tested applicationsELISA, WB, IP, IHC-P, ICC more details
- Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
Porcine brain microtubule alpha Tubulin protein
- Positive control
- Heart tissue.
- Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
- Concentration information loading...
- PurityAmmonium Sulphate Precipitation
- Clonality Monoclonal
- Clone numberTU-16
- Research Areas
Our Abpromise guarantee covers the use of ab78110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||ELISA: Use at an assay dependent dilution.|
|WB||WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 54 kDa.|
|IP||IP: Use a concentration of 1 - 2 µg/ml.|
|IHC-P||IHC-P: Use a concentration of 10 µg/ml.|
|ICC||ICC: Use at an assay dependent dilution.|
- FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
- Sequence similaritiesBelongs to the tubulin family.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
- Cellular localizationCytoplasm > cytoskeleton.
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Anti-alpha Tubulin antibody [TU-16] images
ab78110 at 10µg/ml staining alpha Tubulin in human heart by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue.
A after heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
References for Anti-alpha Tubulin antibody [TU-16] (ab78110)
ab78110 has not yet been referenced specifically in any publications.