Overview

  • Product nameAnti-alpha Tubulin antibody
    See all alpha Tubulin primary antibodies
  • Description
    Rabbit polyclonal to alpha Tubulin
  • Tested applicationsWB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Gerbil, Frog
  • Immunogen

    Synthetic peptide, corresponding to amino acids 426-450 of Human alpha Tubulin. This sequence is identical among Human, Rat, Mouse, Bovine, Guinea pig, Gerbil, Frog and Chicken.

  • Positive control
    • Hela cells; Human lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab125267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
IP Use a concentration of 2 - 5 µg/ml.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha tubulin 2 antibody
    • Alpha tubulin 3 antibody
    • Alpha tubulin 3C/D antibody
    • Alpha-tubulin 1 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • TUBA2 antibody
    • TUBA3 antibody
    • TUBA3C antibody
    • TUBA3D antibody
    • TUBA4A antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha 1A chain antibody
    • Tubulin alpha 2 chain antibody
    • Tubulin alpha 3 chain antibody
    • Tubulin alpha 3C/D chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin B alpha 1 antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • Tubulin, alpha 1a antibody
    • Tubulin, alpha 2 antibody
    • Tubulin, alpha 3 antibody
    • Tubulin, alpha 3c antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, brain specific antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Anti-alpha Tubulin antibody images

  • ab125267 at 1/200 dilution, for 30 minutes at RT, staining alpha Tubulin in formalin-fixed Human lung tissue by Immunohistochemistry.

References for Anti-alpha Tubulin antibody (ab125267)

This product has been referenced in:
  • Ren Y  et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014). WB ; Goat . Read more (PubMed: 24699686) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"