Overview
- Product nameAnti-alpha Tubulin antibodySee all alpha Tubulin primary antibodies ...
- DescriptionRabbit polyclonal to alpha Tubulin
- Tested applicationsWB, IP, IHC-P more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Gerbil, Frog - Immunogen
Synthetic peptide, corresponding to amino acids 426-450 of Human alpha Tubulin. This sequence is identical among Human, Rat, Mouse, Bovine, Guinea pig, Gerbil, Frog and Chicken.
- Positive controlHela cells; Human lung tissue.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.20
Constituent: PBS
Note: Contains antibody stabilizer(0.05% proclin 300,10mg/ml BSA) -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab125267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa. |
| IP | IP: Use a concentration of 2 - 5 µg/ml. |
| IHC-P | IHC-P: 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- RelevanceTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
- Cellular localizationCytoplasmic
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Database links
- Entrez Gene: 404053 Cow
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 7846 Human
- Entrez Gene: 22142 Mouse
- Entrez Gene: 64158 Rat
- Omim: 191110 Human
- Omim: 602529 Human
- Omim: 602528 Human
- SwissProt: P02552 Chicken
- SwissProt: P81947 Cow
- SwissProt: P68366 Human
- SwissProt: Q71U36 Human
- SwissProt: Q13748 Human
- SwissProt: P68369 Mouse
- SwissProt: Q5XJF8 Mouse
- SwissProt: P68370 Rat
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Alternative names
- Alpha tubulin 2 antibodyAlpha tubulin 3 antibodyAlpha tubulin 3C/D antibody
- B ALPHA 1 antibodybA408E5.3 antibodyH2 ALPHA antibodyHum a tub1 antibodyHum a tub2 antibodyLIS3 antibodyTUBA1 antibodyTUBA1A antibodyTUBA2 antibodyTUBA3 antibodyTUBA3C antibodyTUBA3D antibodyTUBA4A antibodyTubulin alpha 1 chain antibodyTubulin alpha 1A chain antibodyTubulin alpha 2 chain antibodyTubulin alpha 3 chain antibodyTubulin alpha 3C/D chain antibodyTubulin alpha antibodyTubulin B alpha 1 antibodyTubulin, alpha 1 (testis specific) antibodyTubulin, alpha 1a antibodyTubulin, alpha 2 antibodyTubulin, alpha 3 antibodyTubulin, alpha 3c antibodyTubulin, alpha 4a antibodyTubulin, alpha, brain specific antibody
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Anti-alpha Tubulin antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody (ab125267)ab125267 at 1/200 dilution, for 30 minutes at RT, staining alpha Tubulin in formalin-fixed Human lung tissue by Immunohistochemistry.
References for Anti-alpha Tubulin antibody (ab125267)
ab125267 has not yet been referenced specifically in any publications.
