Loading...
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Anti-alpha Tubulin antibody
See all alpha Tubulin products (26) ...
Rabbit polyclonal to alpha Tubulin
ICC/IF, IHC-Fr, WB, IHC-P, IPmore details
Reacts with
Mouse, Rat, Human, Pig, Fruit fly (Drosophila melanogaster), Zebrafish
Predicted to work with
Chicken, Guinea pig, Cow, Xenopus laevis, Gerbil, Newt
Synthetic peptide: ALEKDYEEVGVDSVEGEGEEEGEEY conjugated to KLH, corresponding to C terminal amino acids 426-450 of Human alpha Tubulin.
ALEKDYEEVG VDSVEGEGEE EGEEY
Lung tissue slides.
Liquid
Store at +4°C.
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Our Abpromise guarantee covers the use of ab15246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (PubMed: 17785506)
IHC-Fr: Use at an assay dependent dilution. (PubMed: 21069788)
WB: Use at an assay dependent dilution. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P: 1/200Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use at an assay dependent dilution.
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
Cytoplasmic
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Tubulin antibody (ab15246)

ab15246 at 1/200 dilution, staining alpha Tubulin in human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections).
Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody (ab15246)

This image shows a single confocal z-section of a primary porcine macrophage stained with rabbit polyclonal to alpha tubulin.
This picture was kindly supplied as part of the review submitted by Peter Delputte (Laboratory of Virology, Ghent University, Belgium).
Western blot - alpha Tubulin antibody (ab15246)

All lanes : Anti-alpha Tubulin antibody (ab15246) at 1 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 50 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Western blot - alpha Tubulin antibody (ab15246)

Predicted band size : 50 kDa
Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa
Lanes: 1-3: 4oC (1 freeze/thaw); 4-6: 4oC (2 freeze/thaws); 7-9 : 4oC (4 freeze/thaws)
This product has been referenced in:
See all 20 publications for this product
Publishing research using ab15246? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:

ab15246 at 1/200 dilution, staining alpha Tubulin in human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections).

This image shows a single confocal z-section of a primary porcine macrophage stained with rabbit polyclonal to alpha tubulin.
This picture was kindly supplied as part of the review submitted by Peter Delputte (Laboratory of Virology, Ghent University, Belgium).

All lanes : Anti-alpha Tubulin antibody (ab15246) at 1 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 50 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)

Predicted band size : 50 kDa
Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa
Lanes: 1-3: 4oC (1 freeze/thaw); 4-6: 4oC (2 freeze/thaws); 7-9 : 4oC (4 freeze/thaws)
6
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Call 01223 696 000 or contact us
