Overview
- Product nameAnti-alpha Tubulin antibodySee all alpha Tubulin primary antibodies ...
- DescriptionRabbit polyclonal to alpha Tubulin
- Tested applicationsICC, ICC/IF, IHC-Fr, WB, IHC-P, IP more details
- Species reactivityReacts with: Mouse, Rat, Human, Pig, Fruit fly (Drosophila melanogaster), Zebrafish
Predicted to work with: Chicken, Guinea pig, Cow, Xenopus laevis, Gerbil, Newt - Immunogen
Synthetic peptide: ALEKDYEEVGVDSVEGEGEEEGEEY conjugated to KLH, corresponding to C terminal amino acids 426-450 of Human alpha Tubulin.
- Positive controlLung tissue slides.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab15246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC | ICC: Use at an assay dependent concentration. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. PubMed: 17785506 |
| IHC-Fr | IHC-Fr: Use at an assay dependent dilution. PubMed: 21069788 |
| WB | WB: Use at an assay dependent dilution. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa). |
| IHC-P | IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| IP | IP: Use at an assay dependent dilution. |
Target
- RelevanceTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
- Cellular localizationCytoplasmic
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Database links
- Entrez Gene: 404053 Cow
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 7846 Human
- Entrez Gene: 22142 Mouse
- Entrez Gene: 100158003 Pig
- Entrez Gene: 64158 Rat
- Omim: 191110 Human
- Omim: 602529 Human
- Omim: 602528 Human
- SwissProt: P02552 Chicken
- SwissProt: P81947 Cow
- SwissProt: P68366 Human
- SwissProt: Q71U36 Human
- SwissProt: Q13748 Human
- SwissProt: P68369 Mouse
- SwissProt: Q5XJF8 Mouse
- SwissProt: P02550 Pig
- SwissProt: P68370 Rat
see all
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Alternative names
- Alpha tubulin 2 antibodyAlpha tubulin 3 antibodyAlpha tubulin 3C/D antibody
- Alpha tubulin ubiquitous antibodyB ALPHA 1 antibodybA408E5.3 antibodyH2 ALPHA antibodyHum a tub1 antibodyHum a tub2 antibodyLIS3 antibodyTUBA1 antibodyTUBA1A antibodyTUBA2 antibodyTUBA3 antibodyTUBA3C antibodyTUBA3D antibodyTUBA4A antibodyTubulin alpha 1 chain antibodyTubulin alpha 1A chain antibodyTubulin alpha 2 chain antibodyTubulin alpha 3 chain antibodyTubulin alpha 3C/D chain antibodyTubulin alpha antibodyTubulin alpha ubiquitous chain antibodyTubulin B alpha 1 antibodyTubulin K alpha 1 antibodyTubulin, alpha 1 (testis specific) antibodyTubulin, alpha 1a antibodyTubulin, alpha 2 antibodyTubulin, alpha 3 antibodyTubulin, alpha 3c antibodyTubulin, alpha 4a antibodyTubulin, alpha, brain specific antibody
see all
Anti-alpha Tubulin antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Tubulin antibody (ab15246)ab15246 at 1/200 dilution, staining alpha Tubulin in human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections).
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Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody (ab15246)This image shows a single confocal z-section of a primary porcine macrophage stained with rabbit polyclonal to alpha tubulin.
This picture was kindly supplied as part of the review submitted by Peter Delputte (Laboratory of Virology, Ghent University, Belgium).
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Western blot - alpha Tubulin antibody (ab15246)All lanes : Anti-alpha Tubulin antibody (ab15246) at 1 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 50 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?) -
Western blot - alpha Tubulin antibody (ab15246)
Predicted band size : 50 kDa
References for Anti-alpha Tubulin antibody (ab15246)
This product has been referenced in:
- Weil AF et al. Uracil DNA glycosylase initiates degradation of HIV-1 cDNA containing misincorporated dUTP and prevents viral integration. Proc Natl Acad Sci U S A 110:E448-57 (2013). Read more (PubMed: 23341616) »
- Avraham-Davidi I et al. ApoB-containing lipoproteins regulate angiogenesis by modulating expression of VEGF receptor 1. Nat Med : (2012). WB ; Zebrafish . Read more (PubMed: 22581286) »
