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Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Overview
Product name
Anti-alpha Tubulin antibody
See all alpha Tubulin products (26) ...
Description
Rabbit polyclonal to alpha Tubulin
Tested applications
ICC/IF, IHC-Fr, WB, IHC-P, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig, Fruit fly (Drosophila melanogaster), Zebrafish
Predicted to work with
Chicken, Guinea pig, Cow, Xenopus laevis, Gerbil, Newt
Immunogen
Synthetic peptide: ALEKDYEEVGVDSVEGEGEEEGEEY conjugated to KLH, corresponding to C terminal amino acids 426-450 of Human alpha Tubulin.
ALEKDYEEVG VDSVEGEGEE EGEEY
Positive control
Lung tissue slides.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (PubMed: 17785506)
IHC-Fr: Use at an assay dependent dilution. (PubMed: 21069788)
WB: Use at an assay dependent dilution. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P: 1/200Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use at an assay dependent dilution.
Target
Relevance
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
Cellular localization
Cytoplasmic
Alternative names
- Alpha tubulin 2 antibody
- Alpha tubulin 3 antibody
- Alpha tubulin 3C/D antibody
see all
Database links
- Entrez Gene: 404053 Cow
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 7846 Human
- Entrez Gene: 22142 Mouse
- Entrez Gene: 100158003 Pig
- Entrez Gene: 64158 Rat
- Omim: 191110 Human
see all
Anti-alpha Tubulin antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Tubulin antibody (ab15246)
ab15246 at 1/200 dilution, staining alpha Tubulin in human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections).
Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody (ab15246)
This image shows a single confocal z-section of a primary porcine macrophage stained with rabbit polyclonal to alpha tubulin.
This picture was kindly supplied as part of the review submitted by Peter Delputte (Laboratory of Virology, Ghent University, Belgium).
Western blot - alpha Tubulin antibody (ab15246)
All lanes : Anti-alpha Tubulin antibody (ab15246) at 1 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 50 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Western blot - alpha Tubulin antibody (ab15246)
Predicted band size : 50 kDa
Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa
Lanes: 1-3: 4oC (1 freeze/thaw); 4-6: 4oC (2 freeze/thaws); 7-9 : 4oC (4 freeze/thaws)
References for Anti-alpha Tubulin antibody (ab15246)
This product has been referenced in:
- Liang Xet al. NOMPC, a member of the TRP channel family, localizes to the tubular body and distal cilium of Drosophila campaniform and chordotonal receptor cells. Cytoskeleton (Hoboken) 68:1-7 (2011). IHC-Fr; Fruit fly (Drosophila melanogaster).Read more (PubMed: 21069788) »
- Chakrabarti Set al. 17beta-Estradiol induces protein S-nitrosylation in the endothelium. Cardiovasc Res 85:796-805 (2010). WB; Human.Read more (PubMed: 19914929) »
See all 20 publications for this product
Publishing research using ab15246? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"