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Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody (ab18251)
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Western blot - alpha Tubulin antibody (ab18251)
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Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody (ab18251)
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Product Name
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alpha Tubulin antibody
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See all Tubulin (alpha) antibodies (20)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to alpha Tubulin
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.
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Reacts with
(species key)
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Hu, Ms, Chk, ChHm, InMtj
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/2500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
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Relevance
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Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 0.30 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this alpha Tubulin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for alpha Tubulin antibody images, references, products related to ab18251 and other tools.
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alpha Tubulin antibody images:
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 Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody (ab18251)
ICC/IF image of ab18251 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue).
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Western blot - alpha Tubulin antibody (ab18251)
All lanes : alpha Tubulin antibody (ab18251) at 0.5 µg/ml
Lane 1 : HeLa lysate
Lane 2 : A431 lysate
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Flour Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Predicted band size : 50 kDa
Observed band size : 50 kDa
Additional bands at : 30 kDa (possible cross reactivity).
ab18251 detects a strong band at 50 kDa corresponding to alpha tubulin. Cross-reactivity is also seen with other lower molecular weight bands. This may be reduced by using the antibody at a lower working concentration.
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Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody (ab18251)
ab18251 at a 1/8000 dilution staining human HeLa cells by immunocytochemistry. The cells were paraformaldehyde fixed and incubated with the antibody for 30 minutes. The secondary antibody was a Cy3 conjugated Goat Anti-Rabbit IgG (H+L). The image shows staining of an interphase IM cell. This image is courtesy of an Abreview by Kirk McManus submitted on 27 February 2006.
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Tubulin antibody (ab18251)
Image courtesy of Human Protein Atlas ab18251 staining alpha Tubulin. Paraffin embedded human appendix tissue was incubated with ab18251 (1/2500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab18251 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
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References for alpha Tubulin antibody (ab18251)
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This product has been used in: (two most recent references)
Peddibhotla S et al. The DNA-damage effector checkpoint kinase 1 is essential for chromosome segregation and cytokinesis. Proc Natl Acad Sci U S A 106:5159-64 (2009). ICC/IF; Mouse. PubMed: 19289837
Azeloglu EU et al. Atomic force microscope elastography reveals phenotypic differences in alveolar cell stiffness. J Appl Physiol 105:652-61 (2008). PubMed: 18535125
See all 6 publication references for this product.
If you publish research using ab18251 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to alpha Tubulin
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alpha Tubulin antibody - more information
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Customer reviews (feedback) regarding alpha Tubulin antibody |
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alpha Tubulin antibody - related products:
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alpha Tubulin antibody - other tools:
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Contact Abcam with a Technical Enquiry about alpha Tubulin antibody |
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