Anti-alpha Tubulin antibody (ab18251)
- Product nameAnti-alpha Tubulin antibodySee all alpha Tubulin primary antibodies ...
- DescriptionRabbit polyclonal to alpha Tubulin
- Tested applicationsICC/IF, IHC-P, WB more details
- Species reactivityReacts with: Mouse, Rat, Chicken, Human, Fruit fly (Drosophila melanogaster), Indian Muntjac, African Green Monkey, Chinese Hamster
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.
- Positive control
- This antibody gave a positive result in HeLa cells in WB and IF.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab18251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use a concentration of 1 µg/ml.|
|IHC-P||IHC-P: 1/2500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||WB: Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human alpha Tubulin peptide (ab18648).|
- FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
- Sequence similaritiesBelongs to the tubulin family.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
- Cellular localizationCytoplasm > cytoskeleton.
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- TUBA1 antibody
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Anti-alpha Tubulin antibody images
ab18251 staining alpha Tubulin in the COS7 fibroblast cell line from African Green Monkey by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methano and blocked with 5% BSA for 60 minutes at 21°C. Samples were incubated with primary antibody (1/500) for 17 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/400) was used as the secondary antibody.
ICC/IF image of ab18251 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-alpha Tubulin antibody (ab18251) at 0.5 µg/ml
Lane 1 : HeLa lysate
Lane 2 : A431 lysate
Lysates/proteins at 20 µg per lane.
Alexa Flour Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Predicted band size : 50 kDa
Observed band size : 50 kDa
Additional bands at : 30 kDa (possible cross reactivity).
ab18251 detects a strong band at 50 kDa corresponding to alpha tubulin. Cross-reactivity is also seen with other lower molecular weight bands. This may be reduced by using the antibody at a lower working concentration.
ab18251 at a 1/8000 dilution staining human HeLa cells by immunocytochemistry. The cells were paraformaldehyde fixed and incubated with the antibody for 30 minutes. The secondary antibody was a Cy3 conjugated Goat Anti-Rabbit IgG (H+L). The image shows staining of an interphase IM cell.
This image is courtesy of an Abreview by Kirk McManus submitted on 27 February 2006.
Image courtesy of Human Protein Atlas
ab18251 staining alpha Tubulin. Paraffin embedded human appendix tissue was incubated with ab18251 (1/2500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab18251 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
ab18251 staining alpha Tubulin in HeLa cells by Immunocytochemistry/ Immunofluorescence.
ab18251 staining alpha Tubulin labeled red.ab20740 staining Lamin A labeled green.
Hoechst 33258 staining DNA blue.
References for Anti-alpha Tubulin antibody (ab18251)
This product has been referenced in:
- Li M et al. High FSH decreases the developmental potential of mouse oocytes and resulting fertilized embryos, but does not influence offspring physiology and behavior in vitro or in vivo. Hum Reprod 28:1309-23 (2013). Mouse . Read more (PubMed: 23411618) »
- Czeisler C & Mikawa T Microtubules coordinate VEGFR2 signaling and sorting. PLoS One 8:e75833 (2013). Read more (PubMed: 24073279) »