Anti-alpha Tubulin antibody (ab18251)
- Product nameAnti-alpha Tubulin antibodySee all alpha Tubulin primary antibodies ...
- DescriptionRabbit polyclonal to alpha Tubulin
- Tested applicationsICC/IF, IHC-P, WB more details
- Species reactivityReacts with: Mouse, Rat, Chicken, Human, Fruit fly (Drosophila melanogaster), Indian Muntjac, African Green Monkey, Chinese Hamster
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.
- Positive controlThis antibody gave a positive result in HeLa cells in WB and IF.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab18251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use a concentration of 1 µg/ml.|
|IHC-P||IHC-P: 1/2500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||WB: Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with alpha Tubulin peptide (ab18648).|
- RelevanceTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
- Cellular localizationCytoplasmic
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 7846 Human
- Entrez Gene: 22142 Mouse
- Entrez Gene: 64158 Rat
- Omim: 191110 Human
- Omim: 602529 Human
- Omim: 602528 Human
- SwissProt: P02552 Chicken
- SwissProt: P68361 Chinese Hamster
- SwissProt: P68366 Human
- SwissProt: Q71U36 Human
- SwissProt: Q13748 Human
- SwissProt: P68369 Mouse
- SwissProt: Q5XJF8 Mouse
- SwissProt: P68370 Rat
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Anti-alpha Tubulin antibody images
ab18251 staining alpha Tubulin in the COS7 fibroblast cell line from African Green Monkey by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methano and blocked with 5% BSA for 60 minutes at 21°C. Samples were incubated with primary antibody (1/500) for 17 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/400) was used as the secondary antibody.
ICC/IF image of ab18251 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-alpha Tubulin antibody (ab18251) at 0.5 µg/ml
Lane 1 : HeLa lysate
Lane 2 : A431 lysate
Lysates/proteins at 20 µg per lane.
Alexa Flour Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Predicted band size : 50 kDa
Observed band size : 50 kDa
Additional bands at : 30 kDa (possible cross reactivity).
ab18251 detects a strong band at 50 kDa corresponding to alpha tubulin. Cross-reactivity is also seen with other lower molecular weight bands. This may be reduced by using the antibody at a lower working concentration.
ab18251 at a 1/8000 dilution staining human HeLa cells by immunocytochemistry. The cells were paraformaldehyde fixed and incubated with the antibody for 30 minutes. The secondary antibody was a Cy3 conjugated Goat Anti-Rabbit IgG (H+L). The image shows staining of an interphase IM cell.
This image is courtesy of an Abreview by Kirk McManus submitted on 27 February 2006.
Image courtesy of Human Protein Atlas
ab18251 staining alpha Tubulin. Paraffin embedded human appendix tissue was incubated with ab18251 (1/2500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab18251 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
ab18251 staining alpha Tubulin in HeLa cells by Immunocytochemistry/ Immunofluorescence.ab18251 staining alpha Tubulin labeled red.ab20740 staining Lamin A labeled green.Hoechst 33258 staining DNA blue.
References for Anti-alpha Tubulin antibody (ab18251)
This product has been referenced in:
- Li M et al. High FSH decreases the developmental potential of mouse oocytes and resulting fertilized embryos, but does not influence offspring physiology and behavior in vitro or in vivo. Hum Reprod 28:1309-23 (2013). Mouse . Read more (PubMed: 23411618) »
- Kyrkou A et al. RhoD participates in the regulation of cell-cycle progression and centrosome duplication. Oncogene : (2012). Mouse . Read more (PubMed: 22665057) »