For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.
Our Abpromise guarantee covers the use of ab18251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/2500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human alpha Tubulin peptide (ab18648).|
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab18251 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
ab18251 staining alpha Tubulin in the COS7 fibroblast cell line from African Green Monkey by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methano and blocked with 5% BSA for 60 minutes at 21°C. Samples were incubated with primary antibody (1/500) for 17 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/400) was used as the secondary antibody.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab18251 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
ab18251 detects a strong band at 50 kDa corresponding to alpha tubulin. Cross-reactivity is also seen with other lower molecular weight bands. This may be reduced by using the antibody at a lower working concentration.
ab18251 at a 1/8000 dilution staining human HeLa cells by immunocytochemistry. The cells were paraformaldehyde fixed and incubated with the antibody for 30 minutes. The secondary antibody was a Cy3 conjugated Goat Anti-Rabbit IgG (H+L). The image shows staining of an interphase IM cell.
This image is courtesy of an Abreview by Kirk McManus submitted on 27 February 2006.
Image courtesy of Human Protein Atlas
ab18251 staining alpha Tubulin. Paraffin embedded human appendix tissue was incubated with ab18251 (1/2500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab18251 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"