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Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Overview
Product name
Anti-alpha Tubulin antibody
See all alpha Tubulin products (26) ...
Description
Rabbit polyclonal to alpha Tubulin
Tested applications
ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Chicken, Human, Fruit fly (Drosophila melanogaster), Indian Muntjac, Chinese Hamster
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
IHC-P: 1/2500Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB: Use a concentration of 0.5 µg/mlDetects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Target
Relevance
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
Cellular localization
Cytoplasmic
Alternative names
- Alpha tubulin 2 antibody
- Alpha tubulin 3 antibody
- Alpha tubulin 3C/D antibody
see all
Database links
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 7846 Human
- Entrez Gene: 22142 Mouse
- Omim: 191110 Human
- Omim: 602528 Human
- Omim: 602529 Human
- SwissProt: P02552 Chicken
see all
Anti-alpha Tubulin antibody images:
Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody (ab18251)
ICC/IF image of ab18251 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue).
Western blot - alpha Tubulin antibody (ab18251)
All lanes : Anti-alpha Tubulin antibody (ab18251) at 0.5 µg/ml
Lane 1 : HeLa lysate
Lane 2 : A431 lysate
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Flour Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Predicted band size : 50 kDa
Observed band size : 50 kDa
Additional bands at : 30 kDa (possible cross reactivity).
ab18251 detects a strong band at 50 kDa corresponding to alpha tubulin. Cross-reactivity is also seen with other lower molecular weight bands. This may be reduced by using the antibody at a lower working concentration.
Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody (ab18251)
ab18251 at a 1/8000 dilution staining human HeLa cells by immunocytochemistry. The cells were paraformaldehyde fixed and incubated with the antibody for 30 minutes. The secondary antibody was a Cy3 conjugated Goat Anti-Rabbit IgG (H+L). The image shows staining of an interphase IM cell.
This image is courtesy of an Abreview by Kirk McManus submitted on 27 February 2006.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Tubulin antibody (ab18251)
Image courtesy of Human Protein Atlas
ab18251 staining alpha Tubulin. Paraffin embedded human appendix tissue was incubated with ab18251 (1/2500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab18251 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody (ab18251)
ab18251 staining alpha Tubulin in HeLa cells by Immunocytochemistry/ Immunofluorescence.ab18251 staining alpha Tubulin labeled red.ab20740 staining Lamin A labeled green.Hoechst 33258 staining DNA blue.
Image from Mackay DR et al, J Cell Biol. 2010 Nov 29;191(5):923-31. Epub 2010 Nov 22, Fig 1. DOI 10.1083/jcb.201007124
References for Anti-alpha Tubulin antibody (ab18251)
This product has been referenced in:
- Kanakkanthara Aet al. Peloruside- and laulimalide-resistant human ovarian carcinoma cells have ßI-tubulin mutations and altered expression of ßII- and ßIII-tubulin isotypes. Mol Cancer Ther 10:1419-29 (2011).Read more (PubMed: 21653684) »
- Jo Het al. Natural product Celastrol destabilizes tubulin heterodimer and facilitates mitotic cell death triggered by microtubule-targeting anti-cancer drugs. PLoS One 5:e10318 (2010). WB; Human.Read more (PubMed: 20428237) »
See all 11 publications for this product
Publishing research using ab18251? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"