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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using alpha Tubulin antibody (ab61226) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunizing peptide.
All lanes : Anti-alpha Tubulin antibody (ab61226) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract with no immunizing peptide
Lane 2 : NIH/3T3 cell extract with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 50 kDa
Observed band size : 50 kDa
Immunofluorescence analysis of NIH/3T3 cells, using alpha Tubulin antibody (ab61226) at 1/500 - 1/1000 dilution, in the presence (right panel) and absence (left panel) of the immunizing peptide.
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