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Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Overview
Product name
Anti-alpha Tubulin antibody
See all alpha Tubulin products (26) ...
Description
Rabbit polyclonal to alpha Tubulin
Tested applications
ELISA, WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from C-terminal of human alpha Tubulin, (Swiss-Prot Q71U36).
Positive control
NIH/3T3 cells, extracts from NIH/3T3 cells, Human breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
WB: 1/500 - 1/1000.Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P: 1/50 - 1/100.
ICC/IF: 1/500 - 1/1000.
Target
Relevance
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
Cellular localization
Cytoplasmic
Alternative names
- Alpha tubulin 2 antibody
- Alpha tubulin 3 antibody
- Alpha tubulin 3C/D antibody
see all
Database links
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 7846 Human
- Omim: 191110 Human
- Omim: 602528 Human
- Omim: 602529 Human
- SwissProt: P68366 Human
- SwissProt: Q13748 Human
see all
Anti-alpha Tubulin antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Tubulin antibody (ab61226)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using alpha Tubulin antibody (ab61226) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunizing peptide.
Western blot - alpha Tubulin antibody (ab61226)
All lanes : Anti-alpha Tubulin antibody (ab61226) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract with no immunizing peptide
Lane 2 : NIH/3T3 cell extract with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 50 kDa
Observed band size : 50 kDa
Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody (ab61226)
Immunofluorescence analysis of NIH/3T3 cells, using alpha Tubulin antibody (ab61226) at 1/500 - 1/1000 dilution, in the presence (right panel) and absence (left panel) of the immunizing peptide.
References for Anti-alpha Tubulin antibody (ab61226)
ab61226 has not yet been referenced specifically in any publications.
Publishing research using ab61226? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"