Overview

  • Product nameAnti-alpha Tubulin antibody
    See all alpha Tubulin primary antibodies
  • Description
    Rabbit polyclonal to alpha Tubulin
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 277-450 of Human alpha Tubulin (Uniprot ID = Q71U36).

  • Positive control
    • 293T, A431, Jurkat and Raji whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab96059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-tubulin 1 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Anti-alpha Tubulin antibody images

  • All lanes : Anti-alpha Tubulin antibody (ab96059) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa
  • ab96059 at 1/200 dilution staining alpha Tubulin in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The cells in the lower image are costained with Hoechst 33342.

References for Anti-alpha Tubulin antibody (ab96059)

ab96059 has not yet been referenced specifically in any publications.

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