Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
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All lanes : Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) at 1/50000 dilution
Lane 1 : Jurkat cell lysates, untreated.
Lane 2 : Jurkat cell lysates treated with pervanadate.
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size : 54 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
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