![Western blot - alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) image](#ab76290.jpg)
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Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Overview
Product name
Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y]
See all alpha Tubulin products (26) ...
Description
Rabbit monoclonal [EP1334(2)Y] to alpha Tubulin (phospho Y272)
Specificity
This antibody detects alpha Tubulin phosphorylated at tyrosine 272.
Tested applications
WB, ICCmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A phospho synthetic peptide corresponding to residues surrounding tyrosine 272 of human alpha Tubulin.
Positive control
Jurkat cell lysates
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1334(2)Y
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
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Western blot - alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)
![Western blot - alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) image](/ps/datasheet/Images/76/ab76290/ab76290.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/25,000 - 1/50,000.Predicted molecular weight: 54 kDa.
ICC
ICC: 1/50 - 1/100.ICC: 1/50 - 1/100.
Application notes
Is unsuitable for ,Flow Cyt,IHC-P or IP.
Target
Relevance
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
Cellular localization
Cytoplasmic
Alternative names
- Alpha tubulin 2 antibody
- Alpha tubulin 3 antibody
- Alpha tubulin 3C/D antibody
see all
Database links
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 7846 Human
- Omim: 191110 Human
- Omim: 602528 Human
- Omim: 602529 Human
- SwissProt: P68366 Human
- SwissProt: Q13748 Human
see all
Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] images:
Western blot - alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)
All lanes : Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) at 1/50000 dilution
Lane 1 : Jurkat cell lysates, untreated.
Lane 2 : Jurkat cell lysates treated with pervanadate.
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size : 54 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
References for Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)
ab76290 has not yet been referenced specifically in any publications.
Publishing research using ab76290? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"