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Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)

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Overview

Product name

Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y]
See all alpha Tubulin products (26) ...

Description

Rabbit monoclonal [EP1334(2)Y] to alpha Tubulin (phospho Y272)

Specificity

This antibody detects alpha Tubulin phosphorylated at tyrosine 272.

Tested applications

WB, ICCmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A phospho synthetic peptide corresponding to residues surrounding tyrosine 272 of human alpha Tubulin.

Positive control

Jurkat cell lysates

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1334(2)Y

Isotype

IgG

  • Western blot - alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)Western blot - alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/25,000 - 1/50,000....Read more →

    WB: 1/25,000 - 1/50,000.Predicted molecular weight: 54 kDa.

  • ICC

     ICC: 1/50 - 1/100.

    ICC: 1/50 - 1/100.

Application notes

Is unsuitable for ,Flow Cyt,IHC-P or IP.

Target

Relevance

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.

Cellular localization

Cytoplasmic

Alternative names

  • Alpha tubulin 2 antibody
  • Alpha tubulin 3 antibody
  • Alpha tubulin 3C/D antibody
  • B ALPHA 1 antibody
  • bA408E5.3 antibody
  • H2 ALPHA antibody
  • Hum a tub1 antibody
  • Hum a tub2 antibody
  • LIS3 antibody
  • TUBA1 antibody
  • TUBA1A antibody
  • TUBA2 antibody
  • TUBA3 antibody
  • TUBA3C antibody
  • TUBA3D antibody
  • TUBA4A antibody
  • Tubulin alpha 1 chain antibody
  • Tubulin alpha 1A chain antibody
  • Tubulin alpha 2 chain antibody
  • Tubulin alpha 3 chain antibody
  • Tubulin alpha 3C/D chain antibody
  • Tubulin alpha antibody
  • Tubulin B alpha 1 antibody
  • Tubulin K alpha 1 antibody
  • Tubulin, alpha 1 (testis specific) antibody
  • Tubulin, alpha 1a antibody
  • Tubulin, alpha 2 antibody
  • Tubulin, alpha 3 antibody
  • Tubulin, alpha 3c antibody
  • Tubulin, alpha 4a antibody
  • Tubulin, alpha, brain specific antibody
see all

Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] images:

  Western blot - alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)

Western blot - alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)

All lanes : Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) at 1/50000 dilution

Lane 1 : Jurkat cell lysates, untreated.
Lane 2 : Jurkat cell lysates treated with pervanadate.

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size : 54 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)

References for Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)

ab76290 has not yet been referenced specifically in any publications.

Publishing research using ab76290? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"