(2243-1)
Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)
Overview
- Product nameAnti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y]See all alpha Tubulin primary antibodies ...
- DescriptionRabbit monoclonal [EP1334(2)Y] to alpha Tubulin (phospho Y272)
- SpecificityThis antibody detects alpha Tubulin phosphorylated at tyrosine 272.
- Tested applicationsWB, ICC more details
- Species reactivityReacts with: Human
Does not react with
Mouse, Rat - Immunogen
A phospho synthetic peptide corresponding to residues surrounding tyrosine 272 of human alpha Tubulin.
- Positive controlJurkat cell lysates
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEP1334(2)Y
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab76290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/25000 - 1/50000. Predicted molecular weight: 54 kDa. |
| ICC | ICC: 1/50 - 1/100. |
Target
- RelevanceTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
- Cellular localizationCytoplasmic
-
Database links
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 7846 Human
- Omim: 191110 Human
- Omim: 602529 Human
- Omim: 602528 Human
- SwissProt: P68366 Human
- SwissProt: Q71U36 Human
- SwissProt: Q13748 Human
see all
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Alternative names
- Alpha tubulin 2 antibodyAlpha tubulin 3 antibodyAlpha tubulin 3C/D antibody
- B ALPHA 1 antibodybA408E5.3 antibodyH2 ALPHA antibodyHum a tub1 antibodyHum a tub2 antibodyLIS3 antibodyTUBA1 antibodyTUBA1A antibodyTUBA2 antibodyTUBA3 antibodyTUBA3C antibodyTUBA3D antibodyTUBA4A antibodyTubulin alpha 1 chain antibodyTubulin alpha 1A chain antibodyTubulin alpha 2 chain antibodyTubulin alpha 3 chain antibodyTubulin alpha 3C/D chain antibodyTubulin alpha antibodyTubulin B alpha 1 antibodyTubulin K alpha 1 antibodyTubulin, alpha 1 (testis specific) antibodyTubulin, alpha 1a antibodyTubulin, alpha 2 antibodyTubulin, alpha 3 antibodyTubulin, alpha 3c antibodyTubulin, alpha 4a antibodyTubulin, alpha, brain specific antibody
see all
Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] images
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![Western blot - alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)](http://a.abcam.com/ps/datasheet/Images/76/ab76290/ab76290.jpg)
Western blot - alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)All lanes : Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) at 1/50000 dilution
Lane 1 : Jurkat cell lysates, untreated.
Lane 2 : Jurkat cell lysates treated with pervanadate.
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size : 54 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
References for Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)
This product has been referenced in:
- Meshki J et al. Substance P induces rapid and transient membrane blebbing in U373MG cells in a p21-activated kinase-dependent manner. PLoS One 6:e25332 (2011). WB . Read more (PubMed: 21966499) »
