alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)

Product Name 

alpha Tubulin (phospho Y272) antibody [EP1334(2)Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1334(2)Y] to alpha Tubulin (phospho Y272)

Immunogen 

A phospho synthetic peptide corresponding to residues surrounding tyrosine 272 of human alpha Tubulin.

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Specificity 

This antibody detects alpha Tubulin phosphorylated at tyrosine 272.

Tested applications 
(see key)

ICC, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: 1/50 - 1/100.
WB: 1/25,000 - 1/50,000. Predicted molecular weight: 54 kDa.

Is unsuitable for Flow Cyt, IHC-P or IP.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat cell lysates

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules

Relevance 

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP1334(2)Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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