Anti-Alpha A + alpha B Crystallin antibody (ab28163)

Overview

  • Product nameAnti-Alpha A + alpha B Crystallin antibody
    See all Alpha A + alpha B Crystallin primary antibodies
  • Description
    Rabbit polyclonal to Alpha A + alpha B Crystallin
  • Specificityab28163 recognises alpha A and alpha B crystallin proteins. There is no reactivity with beta crystallin.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Synthetic peptides:

    EEKPTSAPSS

    conjugated to KLH, corresponding to amino acids 164/173 of Human alpha A; and REEKPAVTAAPKK conjugated to KLH, corresponding to amino acids 163/175 of Human alpha B Crystallin

  • Positive control
    • Bovine alpha Crystallin Protein, Bovine alpha A Crystallin Protein, Bovine alpha B Crystallin Protein

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab28163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/1000. This dilution was sufficient for detection of alpha A/beta B-crystallin in 20µg of alpha A-crystallin protein by colorimetric immunoblot analysis Goat anti-Rabbit IgG-AP as secondary antibody. Predicted molecular weight: 20 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCrystallins are separated into two classes: taxon specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Alpha crystallins are composed of two gene products: alpha A and alpha B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • Alpha crystallin A chain antibody
      • Alpha crystallin B chain antibody
      • CRYA1 antibody
      • CRYA2 antibody
      • CRYAA antibody
      • CRYAB antibody
      • Heat shock protein beta 4 antibody
      • Heat shock protein beta 5 antibody
      • HspB4 antibody
      • HspB5 antibody
      • Rosenthal fiber component antibody
      see all

    Anti-Alpha A + alpha B Crystallin antibody images

    • All lanes : Anti-Alpha A + alpha B Crystallin antibody (ab28163) at 1/1000 dilution

      Lane 1 : Tissue lysates prepared from mouse heart
      Lane 2 : Cell lysates prepared from Hela cells
      Lane 3 : Crystallin alpha B native bovine protein
      Lane 4 : Crystallin alpha native bovine protein
      Lane 5 : Molecular weight marker


      Predicted band size : 20 kDa

    References for Anti-Alpha A + alpha B Crystallin antibody (ab28163)

    ab28163 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"