Anti-Alpha A + alpha B Crystallin antibody (ab28163)

Overview

  • Product name
    Anti-Alpha A + alpha B Crystallin antibody
    See all Alpha A + alpha B Crystallin primary antibodies
  • Description
    Rabbit polyclonal to Alpha A + alpha B Crystallin
  • Specificity
    ab28163 recognises alpha A and alpha B crystallin proteins. There is no reactivity with beta crystallin.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Synthetic peptides:

    EEKPTSAPSS

    conjugated to KLH, corresponding to amino acids 164/173 of Human alpha A; and REEKPAVTAAPKK conjugated to KLH, corresponding to amino acids 163/175 of Human alpha B Crystallin

  • Positive control
    • Bovine alpha Crystallin Protein, Bovine alpha A Crystallin Protein, Bovine alpha B Crystallin Protein

Properties

Applications

Our Abpromise guarantee covers the use of ab28163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 20 kDa.

This dilution was sufficient for detection of alpha A/beta B-crystallin in 20µg of alpha A-crystallin protein by colorimetric immunoblot analysis. Goat anti-Rabbit IgG-AP as secondary antibody.

ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Crystallins are separated into two classes: taxon specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Alpha crystallins are composed of two gene products: alpha A and alpha B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • Alpha crystallin A chain antibody
    • Alpha crystallin B chain antibody
    • CRYA1 antibody
    • CRYA2 antibody
    • CRYAA antibody
    • CRYAB antibody
    • Heat shock protein beta 4 antibody
    • Heat shock protein beta 5 antibody
    • HspB4 antibody
    • HspB5 antibody
    • Rosenthal fiber component antibody
    see all

Anti-Alpha A + alpha B Crystallin antibody images

  • All lanes : Anti-Alpha A + alpha B Crystallin antibody (ab28163) at 1/1000 dilution

    Lane 1 : Tissue lysates prepared from mouse heart
    Lane 2 : Cell lysates prepared from Hela cells
    Lane 3 : Crystallin alpha B native bovine protein
    Lane 4 : Crystallin alpha native bovine protein
    Lane 5 : Molecular weight marker


    Predicted band size : 20 kDa

References for Anti-Alpha A + alpha B Crystallin antibody (ab28163)

ab28163 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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