Anti-alpha Actinin 4 antibody - Carboxyterminal end (ab82365)


  • Product nameAnti-alpha Actinin 4 antibody - Carboxyterminal end
    See all alpha Actinin 4 primary antibodies
  • Description
    Rabbit polyclonal to alpha Actinin 4 - Carboxyterminal end
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human alpha Actinin 4 aa 792-821 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • HepG2 cytoplasmic or nuclear lysate Mouse bladder cytoplasmic or nuclear lysate



Our Abpromise guarantee covers the use of ab82365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).


  • FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseDefects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1) [MIM:603278]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similaritiesBelongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines.
  • Information by UniProt
  • Database links
  • Alternative names
    • actinin 4 antibody
    • Actinin alpha 4 antibody
    • actinin4 antibody
    • ACTN 4 antibody
    • ACTN4 antibody
    • ACTN4_HUMAN antibody
    • Alpha-actinin-4 antibody
    • DKFZp686K23158 antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    • Focal segmental glomerulosclerosis 1 antibody
    • FSGS 1 antibody
    • FSGS antibody
    • FSGS1 antibody
    • Non muscle alpha actinin 4 antibody
    • Non-muscle alpha-actinin 4 antibody
    see all

Anti-alpha Actinin 4 antibody - Carboxyterminal end images

  • Flow cytometric analysis of MDA-231 cells using ab82365(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ab82365, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Lane 1 :
    Lane 2 :
    Lane 3 :
    Lane 4 :
    Lane 5 :

    Lane 1 : T47D cell Lysate
    Lane 2 : A2058 cell Lysate
    Lane 3 : A375 cell Lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : Mouse bladder tissue lysates

    Lysates/proteins at 35 µg per lane.

    Predicted band size : 105 kDa

References for Anti-alpha Actinin 4 antibody - Carboxyterminal end (ab82365)

ab82365 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab82365.
Please use the links above to contact us or submit feedback about this product.