Anti-alpha Actinin 4 antibody [EPR2533(2)] (ab108198)

Overview

  • Product nameAnti-alpha Actinin 4 antibody [EPR2533(2)]
    See all alpha Actinin 4 primary antibodies
  • Description
    Rabbit monoclonal [EPR2533(2)] to alpha Actinin 4
  • Tested applicationsSuitable for: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the N-terminus of Human alpha Actinin 4.

  • Positive control
    • Fetal skeletal muscle, A431, MCF-7, and HeLa cell lysates; Human kidney tissue; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab108198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 105 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.

Target

  • FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseDefects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1) [MIM:603278]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similaritiesBelongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines.
  • Information by UniProt
  • Database links
  • Alternative names
    • actinin 4 antibody
    • Actinin alpha 4 antibody
    • actinin4 antibody
    • ACTN 4 antibody
    • ACTN4 antibody
    • ACTN4_HUMAN antibody
    • Alpha-actinin-4 antibody
    • DKFZp686K23158 antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    • Focal segmental glomerulosclerosis 1 antibody
    • FSGS 1 antibody
    • FSGS antibody
    • FSGS1 antibody
    • Non muscle alpha actinin 4 antibody
    • Non-muscle alpha-actinin 4 antibody
    see all

Anti-alpha Actinin 4 antibody [EPR2533(2)] images

  • ab108198 staining alpha Actinin 4 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/50. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • All lanes : Anti-alpha Actinin 4 antibody [EPR2533(2)] (ab108198) at 1/1000 dilution

    Lane 1 : Fetal skeletal muscle lysate
    Lane 2 : A431 cell lysate
    Lane 3 : MCF-7 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 105 kDa
  • ab108198, at 1/100 dilution, staining alpha Actinin 4 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab108198, at 1/100 dilution, staining alpha Actinin 4 in HeLa cells by Immunofluorescence.

References for Anti-alpha Actinin 4 antibody [EPR2533(2)] (ab108198)

This product has been referenced in:
  • Fu X  et al. Doublecortin (Dcx) family proteins regulate filamentous actin structure in developing neurons. J Neurosci 33:709-21 (2013). Mouse . Read more (PubMed: 23303949) »
  • Bailey CK  et al. High Motility of Triple-negative Breast Cancer Cells Is Due to Repression of Plakoglobin Gene by Metastasis Modulator Protein SLUG. J Biol Chem 287:19472-86 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22496452) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Vascular smooth muscle cells)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Specification Vascular smooth muscle cells
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Dec 02 2016

Application Western blot
Sample Rat Cell lysate - other (Primary lung epithelia cells)
Gel Running Conditions Reduced Denaturing (4-12% Nupage)
Specification Primary lung epithelia cells
Blocking step (agent) for 2 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C
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Submitted Nov 02 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Breast)
Loading amount 20 µg
Specification Breast
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Breast)
Specification Breast
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Aug 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"