Anti-alpha Actinin antibody [0.T.02] (ab18061)

Overview

  • Product nameAnti-alpha Actinin antibody [0.T.02]
    See all alpha Actinin primary antibodies
  • Description
    Mouse monoclonal [0.T.02] to alpha Actinin
  • SpecificityRecognizes and is specific for human alpha Actinin 1 isoform (non-muscle) at 100kD. This antibody may recognize other isoforms of alpha-actinin (1-4). There is no specific epitope information available.
  • Tested applicationsSuitable for: WB, IHC-Fr, IHC (Methanol fixed), IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Sheep, Rabbit, Human
  • Immunogen

    Human alpha Actinin.

  • Positive control
    • RIPA lysates from human A431 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab18061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-Fr
IHC (Methanol fixed)
IHC-P
ICC/IF
  • Application notesICC/IF: Use at a concentration of 15 µg/ml.
    IHC-Fr: Use at a concentration of 15 µg/ml.
    Immunohistochemistry (Methanol fixed): Use at a concentration of 15 µg/ml.

    WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 103 kDa.

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
    • Sequence similaritiesBelongs to the alpha-actinin family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
      Contains 4 spectrin repeats.
    • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
    • Information by UniProt
    • Database links
    • Alternative names
      • actinin 1 smooth muscle antibody
      • Actinin alpha 1 antibody
      • actinin, alpha 1 antibody
      • ACTN 1 antibody
      • Actn1 antibody
      • ACTN1_HUMAN antibody
      • Alpha Actinin 1 antibody
      • Alpha actinin cytoskeletal isoform antibody
      • Alpha-actinin cytoskeletal isoform antibody
      • Alpha-actinin-1 antibody
      • BDPLT15 antibody
      • F actin cross linking protein antibody
      • F-actin cross-linking protein antibody
      • FLJ40884 antibody
      • FLJ54432 antibody
      • Non muscle alpha actinin 1 antibody
      • Non-muscle alpha-actinin-1 antibody
      see all

    Anti-alpha Actinin antibody [0.T.02] images

    • Anti-alpha Actinin antibody [0.T.02] (ab18061) at 1/2000 dilution + Human pancreatic cancer cell line - whole cell lysate at 20 µg

      Secondary
      HRP conjugated goat anti-mouse antibody at 1/2000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 103 kDa
      Observed band size : 103 kDa


      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

      See Abreview

    • ICC/IF image of ab18061 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18061, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-alpha Actinin antibody [0.T.02] (ab18061)

    This product has been referenced in:
    • Ngan E  et al. A complex containing LPP and a-actinin mediates TGFß-induced migration and invasion of ErbB2-expressing breast cancer cells. J Cell Sci 126:1981-91 (2013). Read more (PubMed: 23447672) »
    • Indra I  et al. The Adherens Junction: A Mosaic of Cadherin and Nectin Clusters Bundled by Actin Filaments. J Invest Dermatol N/A:N/A (2013). Human . Read more (PubMed: 23639974) »

    See all 9 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (mouse skin tumor)
    Specification mouse skin tumor
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6.0
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 15 2013

    We now have new lot in stock so I can send you one vial as a replacement antibody. Again because we haven't tested this lot in mouse so we cannot confirm the ab will work.

    You have kindly mentioned in previous emails that the antibody worked ...

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    Thank you for confirming all the details.

    The mouse species has been added to the datasheet from a publication http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20362548&dopt=Abstract. We unfortunately...

    Read More

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    I a...

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    Thank you for contacting us. There are many proteins that act as markers for Cardiomyocytes; antibodies against these targets can be used for ICC/IF or other required applications. Main cardiac proteins are as follows; - alpha actin; alpha s...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Sheep Tissue lysate - whole (Whole Placentome)
    Loading amount 100 µg
    Specification Whole Placentome
    Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Bryan White

    Verified customer

    Submitted May 11 2011

    Thank you for your enquiry. The immunogen for ab18061 is full-length human alpha-actinin 1 from platelets. There is no epitope information for this monoclonal antibody; however, the sequence homology between human, mouse and rat alpha-actinin 1 is 99%...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Lung carcinoma)
    Loading amount 40 µg
    Specification Lung carcinoma
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 24 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (pancreatic cancer cell line)
    Loading amount 20 µg
    Specification pancreatic cancer cell line
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 09 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (cardiomyocyte)
    Specification cardiomyocyte
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10%
    Username

    Abcam user community

    Verified customer

    Submitted Feb 09 2007

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"