Overview

  • Product name
    Anti-alpha Actinin antibody
    See all alpha Actinin primary antibodies
  • Description
    Rabbit polyclonal to alpha Actinin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 220-380 of Human alpha Actinin (Uniprot ID: P12814).

  • Positive control
    • 293T, A431, H1299, HeLa S3, HepG2, MOLT4, NIH 3T3 and Raji whole cell lysates. Human SW480 xenograft tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 103 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

Images

  • Anti-alpha Actinin antibody (ab155480) at 1/1000 dilution + mouse NIH 3T3 whole cell lysate at 30 µg

    Predicted band size : 103 kDa
  • All lanes : Anti-alpha Actinin antibody (ab155480) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa S3 whole cell lysate
    Lane 4 : HepG2 whole cell lysate
    Lane 5 : MOLT4 whole cell lysate
    Lane 6 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 103 kDa
  • Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue, labeling alpha Actinin using ab155480 at a 1/100 dilution.

References

ab155480 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155480.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up