Overview

  • Product name
    Anti-alpha Actinin antibody
    See all alpha Actinin primary antibodies
  • Description
    Goat polyclonal to alpha Actinin
  • Specificity
    ab190872 is expected to recognize all reported isoforms (NP_001123476.1; NP_001093.1; NP_001123477.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human alpha Actinin aa 596-609 (Cysteine residue).
    Sequence:

    TPQEINGKWDHVRQ


    Database link: P12814

  • Positive control
    • Human spleen lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab190872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 103 kDa).

Target

Images

  • All lanes : Anti-alpha Actinin antibody (ab190872) at 0.3 µg/ml (Incubation time: 1 hour)

    Lane 1 : Mouse spleen Lysates in RIPA buffer
    Lane 2 : Rat spleen Lysates in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 103 kDa
  • Anti-alpha Actinin antibody (ab190872) at 0.3 µg/ml + Human spleen lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 103 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Primary incubation was 1 hour.

References

ab190872 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab190872.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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