Anti-alpha Actinin antibody [AT6/172] (ab18061)

Overview

  • Product name
    Anti-alpha Actinin antibody [AT6/172]
    See all alpha Actinin primary antibodies
  • Description
    Mouse monoclonal [AT6/172] to alpha Actinin
  • Specificity
    Recognizes and is specific for human alpha Actinin 1 isoform (non-muscle) at 100 kDa. This antibody may recognize other isoforms of alpha-actinin (1-4). There is no specific epitope information available. The clone number has been updated from (0.T.02) to (AT6/172) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC (Methanol fixed), IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Sheep, Rabbit, Human
  • Immunogen

    Full length protein corresponding to Human alpha Actinin.
    Database link: P12814

  • Positive control
    • WB: Human pancreatic cancer whole cell lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab18061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 103 kDa.
IHC-Fr Use a concentration of 15 µg/ml.
IHC (Methanol fixed) Use a concentration of 15 µg/ml.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 15 µg/ml.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
    • Sequence similarities
      Belongs to the alpha-actinin family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
      Contains 4 spectrin repeats.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
    • Information by UniProt
    • Database links
    • Alternative names
      • actinin 1 smooth muscle antibody
      • Actinin alpha 1 antibody
      • actinin, alpha 1 antibody
      • ACTN 1 antibody
      • Actn1 antibody
      • ACTN1_HUMAN antibody
      • Alpha Actinin 1 antibody
      • Alpha actinin cytoskeletal isoform antibody
      • Alpha-actinin cytoskeletal isoform antibody
      • Alpha-actinin-1 antibody
      • BDPLT15 antibody
      • F actin cross linking protein antibody
      • F-actin cross-linking protein antibody
      • FLJ40884 antibody
      • FLJ54432 antibody
      • Non muscle alpha actinin 1 antibody
      • Non-muscle alpha-actinin-1 antibody
      see all

    Images

    • Anti-alpha Actinin antibody [AT6/172] (ab18061) at 1/2000 dilution + Human pancreatic cancer cell line - whole cell lysate at 20 µg

      Secondary
      HRP conjugated goat anti-mouse antibody at 1/2000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 103 kDa
      Observed band size : 103 kDa


      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

      See Abreview

    • ICC/IF image of ab18061 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3 M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18061, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

    References

    This product has been referenced in:
    • Zheng C  et al. Gastrodin Inhibits Store-Operated Ca(2+) Entry and Alleviates Cardiac Hypertrophy. Front Pharmacol 8:222 (2017). ICC/IF ; Mouse . Read more (PubMed: 28487655) »
    • Han X  et al. Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules. Nat Commun 8:14639 (2017). Read more (PubMed: 28256511) »

    See all 15 Publications for this product

    Customer reviews and Q&As

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse skin tumor)
    Specification
    mouse skin tumor
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 15 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Whole Placentome)
    Loading amount
    100 µg
    Specification
    Whole Placentome
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Bryan White

    Verified customer

    Submitted May 11 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lung carcinoma)
    Loading amount
    40 µg
    Specification
    Lung carcinoma
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 24 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (pancreatic cancer cell line)
    Loading amount
    20 µg
    Specification
    pancreatic cancer cell line
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 09 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (cardiomyocyte)
    Specification
    cardiomyocyte
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10%
    Username

    Abcam user community

    Verified customer

    Submitted Feb 09 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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