Anti-alpha Actinin antibody [BM 75.2] (ab11008)

Overview

  • Product nameAnti-alpha Actinin antibody [BM 75.2]See all alpha Actinin primary antibodies ...
  • Description
    Mouse monoclonal [BM 75.2] to alpha Actinin
  • SpecificityMonoclonal anti-alpha Actinin specifically stains alpha actinin in cell cultures using indirect immunofluorescent labeling.
  • Tested applicationsDouble Immunodiffusion, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human
  • Immunogen

    Cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells.

  • Positive control
    • chicken fibroblasts
  • General notesIf slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Double Immunodiffusion Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 107 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • Sequence similaritiesBelongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
  • Information by UniProt
  • Database links
  • Alternative names
    • actinin 1 smooth muscle antibody
    • Actinin alpha 1 antibody
    • actinin, alpha 1 antibody
    • ACTN 1 antibody
    • ACTN1 antibody
    • ACTN1_HUMAN antibody
    • Alpha Actinin 1 antibody
    • Alpha actinin cytoskeletal isoform antibody
    • Alpha-actinin cytoskeletal isoform antibody
    • Alpha-actinin-1 antibody
    • BDPLT15 antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    • FLJ40884 antibody
    • FLJ54432 antibody
    • Non muscle alpha actinin 1 antibody
    • Non-muscle alpha-actinin-1 antibody
    see all

Anti-alpha Actinin antibody [BM 75.2] images

  • Anti-alpha Actinin antibody [BM 75.2] (ab11008) (Human whole cell lysate (MDA MB 231 cells))

    Secondary
    Sheep anti-mouse HRP

    Predicted band size : 107 kDa
    Observed band size : 115 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab11008 staining alpha Actinin in Human fibrosarcoma cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton. Samples were incubated with 10 µg/ml primary antibody in PBS for 1 hour at  20°C.  An Alexa Fluor® -conjugated Donkey monoclonal to mouse IgG, dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI.

    See Abreview

References for Anti-alpha Actinin antibody [BM 75.2] (ab11008)

This product has been referenced in:
  • Calla-Choque JS  et al. a -Actinin TvACTN3 of Trichomonas vaginalis is an RNA-binding protein that could participate in its posttranscriptional iron regulatory mechanism. Biomed Res Int 2014:424767 (2014). Read more (PubMed: 24719864) »
  • Averbeck M  et al. Switch in syndecan-1 and syndecan-4 expression controls maturation associated dendritic cell motility. Exp Dermatol 16:580-9 (2007). Read more (PubMed: 17576238) »

See all 4 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibrosarcoma)
Specification fibrosarcoma
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
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Verified customer

Submitted Oct 19 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount 10 µg
Specification NIH 3T3 cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Mar 24 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MDA MB 231 cells)
Loading amount 10 µg
Specification MDA MB 231 cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 24 2009

Thank you for your telephone enquiry this morning. This antibody was raised using the cytoskeletal fraction of bovine mammary gland epithelial (BMGE) cells. We like to make data available to customers on the epitope for antibodies whenever possible, bu...

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I have been unsuccessful at finding out if our antibodies against ACTN (Ab11007, ab1108) were specific to ACTN1 or ACTN4, my sincere apologies. Unfortunately we currently do not have in our catalogue antibodies that match your requirements, may I sugge...

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Thank you for your enquiry. We have not specifically assayed to see if any of these antibodies reacts with alpha-actinin 2. However,the immunogens used to create ab11007 and ab11008 were whole extracts from either chicken gizzard or BMGE cells. Thes...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"