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Synthetic peptide within Human alpha Actinin aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P12814
Our Abpromise guarantee covers the use of ab175944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 103 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling alpha Actinin with ab175944 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of MDA-231 cells labeling alpha Actinin with ab175944 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab175944 has not yet been referenced specifically in any publications.