Overview

  • Product name
    Anti-alpha Adaptin antibody
    See all alpha Adaptin primary antibodies
  • Description
    Rabbit polyclonal to alpha Adaptin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human alpha Adaptin.
    Database link: O94973

  • Positive control
    • 22RV1, SK0V3 and Mouse brain lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 104 kDa.
IHC-P 1/50 - 1/200.

Target

  • Relevance
    Clathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • 100 kDa coated vesicle protein A antibody
    • 100 kDa coated vesicle protein C antibody
    • Adapter related protein complex 2 subunit alpha 1 antibody
    • Adapter related protein complex 2 subunit alpha 2 antibody
    • Adaptin alpha A antibody
    • Adaptin alpha B antibody
    • Adaptin, alpha B antibody
    • Adaptor protein complex AP 2 alpha 1 subunit antibody
    • Adaptor protein complex AP 2 alpha 2 subunit antibody
    • Adaptor protein complex AP 2 subunit alpha 1 antibody
    • Adaptor related protein complex 2 alpha 1 subunit antibody
    • Adaptor related protein complex 2 alpha 2 subunit antibody
    • ADTAA antibody
    • ADTAB antibody
    • Alpha adaptin A antibody
    • Alpha adaptin C antibody
    • Alpha adaptin C; Huntingtin interacting protein J antibody
    • Alpha1 adaptin antibody
    • Alpha2 adaptin antibody
    • AP 2 complex subunit alpha 1 antibody
    • AP 2 complex subunit alpha 2 antibody
    • AP2 alpha antibody
    • AP2A1 antibody
    • AP2A2 antibody
    • CLAPA1 antibody
    • CLAPA2 antibody
    • Clathrin assembly protein complex 2 alpha A large chain antibody
    • Clathrin assembly protein complex 2 alpha C large chain antibody
    • Clathrin associated/assembly/adaptor protein large alpha 1 antibody
    • Clathrin associated/assembly/adaptor protein large alpha 2 antibody
    • HIP 9 antibody
    • HIP9 antibody
    • Huntingtin interacting protein antibody
    • Huntingtin interacting protein HYPJ antibody
    • Huntingtin interacting protein J antibody
    • Huntingtin yeast partner J antibody
    • HYPJ antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody
    see all

Images

  • All lanes : Anti-alpha Adaptin antibody (ab186828) at 1/500 dilution

    Lane 1 : 22RV1 cell extracts
    Lane 2 : SK0V3 cell extracts
    Lane 3 : Mouse brain cell extracts

    Predicted band size: 104 kDa

References

ab186828 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab186828.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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