Overview

  • Product nameAnti-alpha Adaptin antibody
    See all alpha Adaptin primary antibodies
  • Description
    Rabbit polyclonal to alpha Adaptin
  • Specificityab91268 detects the AP1 adaptor of alpha Adaptin.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the center region of Human alpha Adaptin, conjugated to KLH.

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab91268 is purified through a Protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab91268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 104 kDa (for AP2 of alpha Adaptin).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceClathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • 100 kDa coated vesicle protein A antibody
      • 100 kDa coated vesicle protein C antibody
      • Adapter related protein complex 2 subunit alpha 1 antibody
      • Adapter related protein complex 2 subunit alpha 2 antibody
      • Adaptin alpha A antibody
      • Adaptin alpha B antibody
      • Adaptin, alpha B antibody
      • Adaptor protein complex AP 2 alpha 1 subunit antibody
      • Adaptor protein complex AP 2 alpha 2 subunit antibody
      • Adaptor protein complex AP 2 subunit alpha 1 antibody
      • Adaptor related protein complex 2 alpha 1 subunit antibody
      • Adaptor related protein complex 2 alpha 2 subunit antibody
      • ADTAA antibody
      • ADTAB antibody
      • Alpha adaptin A antibody
      • Alpha adaptin C antibody
      • Alpha adaptin C; Huntingtin interacting protein J antibody
      • Alpha1 adaptin antibody
      • Alpha2 adaptin antibody
      • AP 2 complex subunit alpha 1 antibody
      • AP 2 complex subunit alpha 2 antibody
      • AP2 alpha antibody
      • AP2A1 antibody
      • AP2A2 antibody
      • CLAPA1 antibody
      • CLAPA2 antibody
      • Clathrin assembly protein complex 2 alpha A large chain antibody
      • Clathrin assembly protein complex 2 alpha C large chain antibody
      • Clathrin associated/assembly/adaptor protein large alpha 1 antibody
      • Clathrin associated/assembly/adaptor protein large alpha 2 antibody
      • HIP 9 antibody
      • HIP9 antibody
      • Huntingtin interacting protein antibody
      • Huntingtin interacting protein HYPJ antibody
      • Huntingtin interacting protein J antibody
      • Huntingtin yeast partner J antibody
      • HYPJ antibody
      • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
      • Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody
      see all

    Anti-alpha Adaptin antibody images

    • Anti-alpha Adaptin antibody (ab91268) at 1/100 dilution + HepG2 cell lysate at 35 µg

      Predicted band size : 104 kDa

    References for Anti-alpha Adaptin antibody (ab91268)

    ab91268 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab91268.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"