Anti-alpha Adaptin antibody [AC1-M11] (ab2807)

Overview

  • Product nameAnti-alpha Adaptin antibody [AC1-M11]
    See all alpha Adaptin primary antibodies
  • Description
    Mouse monoclonal [AC1-M11] to alpha Adaptin
  • Tested applicationsSuitable for: IP, WB, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human, Pig, Non Human Primates, Amphibians
  • Immunogen

    Full length native protein (purified) corresponding to Cow alpha Adaptin. Purified Bovine brain adaptor complexes

  • Positive control
    • pig brain lysate and transfected COS cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberAC1-M11
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/100. Detects a band of approximately 100 kDa. In pig brain extract detects a doublet of approximately 100 kDa representing the two isoforms of alpha-adaptin.
ICC/IF 1/100.
ELISA Use at an assay dependent concentration.
IHC-P 1/20.
Flow Cyt 1/100.

Target

  • RelevanceClathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • 100 kDa coated vesicle protein A antibody
    • 100 kDa coated vesicle protein C antibody
    • Adapter related protein complex 2 subunit alpha 1 antibody
    • Adapter related protein complex 2 subunit alpha 2 antibody
    • Adaptin alpha A antibody
    • Adaptin alpha B antibody
    • Adaptin, alpha B antibody
    • Adaptor protein complex AP 2 alpha 1 subunit antibody
    • Adaptor protein complex AP 2 alpha 2 subunit antibody
    • Adaptor protein complex AP 2 subunit alpha 1 antibody
    • Adaptor related protein complex 2 alpha 1 subunit antibody
    • Adaptor related protein complex 2 alpha 2 subunit antibody
    • ADTAA antibody
    • ADTAB antibody
    • Alpha adaptin A antibody
    • Alpha adaptin C antibody
    • Alpha adaptin C; Huntingtin interacting protein J antibody
    • Alpha1 adaptin antibody
    • Alpha2 adaptin antibody
    • AP 2 complex subunit alpha 1 antibody
    • AP 2 complex subunit alpha 2 antibody
    • AP2 alpha antibody
    • AP2A1 antibody
    • AP2A2 antibody
    • CLAPA1 antibody
    • CLAPA2 antibody
    • Clathrin assembly protein complex 2 alpha A large chain antibody
    • Clathrin assembly protein complex 2 alpha C large chain antibody
    • Clathrin associated/assembly/adaptor protein large alpha 1 antibody
    • Clathrin associated/assembly/adaptor protein large alpha 2 antibody
    • HIP 9 antibody
    • HIP9 antibody
    • Huntingtin interacting protein antibody
    • Huntingtin interacting protein HYPJ antibody
    • Huntingtin interacting protein J antibody
    • Huntingtin yeast partner J antibody
    • HYPJ antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody
    see all

Anti-alpha Adaptin antibody [AC1-M11] images

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human heart tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing alpha Adaptin (ab2807) or without primary antibody (negative control) overnight at 4�C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Immunolocalization of alpha-adaptin in NRK cells using ab2807.
  • Immunolocalization of alpha-adaptin in NRK cells using ab2807 (low power image of image1).
  • Anti-alpha Adaptin antibody [AC1-M11] (ab2807) at 1/500 dilution + Lysate was prepared from mouse neuroblastoma cells at 10 µg

    Secondary
    HRP-conjugated donkey monoclonal to mouse IgG at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is a courtesy of Anonymous Abreview

    See Abreview

  • Overlay histogram showing HepG2 cells stained with ab2807 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2807, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-alpha Adaptin antibody [AC1-M11] (ab2807)

This product has been referenced in:
  • Gautier JJ  et al. Clathrin is required for Scar/Wave-mediated lamellipodium formation. J Cell Sci 124:3414-27 (2011). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 22010197) »
  • Robinson MS 100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies. J Cell Biol 104:887-95 (1987). Read more (PubMed: 2881934) »

See all 2 Publications for this product

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The concentration of this lot is 2mg/ml.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Neuroblastoma)
Loading amount 10 µg
Specification Neuroblastoma
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Nov 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"