Anti-alpha Adducin antibody (ab54985)

Overview

  • Product nameAnti-alpha Adducin antibody
    See all alpha Adducin primary antibodies
  • Description
    Mouse monoclonal to alpha Adducin
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-663 of Human alpha Adducin

Properties

Applications

Our Abpromise guarantee covers the use of ab54985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 81 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMembrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
  • Tissue specificityExpressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
  • Sequence similaritiesBelongs to the aldolase class II family. Adducin subfamily.
  • DomainEach subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
  • Cellular localizationCytoplasm > cytoskeleton. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADD A antibody
    • ADD1 antibody
    • ADDA antibody
    • ADDA_HUMAN antibody
    • Adducin 1 (alpha) antibody
    • Alpha adducin antibody
    • Alpha-adducin antibody
    • Erythrocyte adducin alpha subunit antibody
    • Erythrocyte adducin subunit alpha antibody
    • MGC3339 antibody
    • MGC44427 antibody
    • OTTHUMP00000151163 antibody
    • OTTHUMP00000151164 antibody
    • OTTHUMP00000151165 antibody
    • OTTHUMP00000218664 antibody
    • OTTHUMP00000218665 antibody
    see all

Anti-alpha Adducin antibody images

  • alpha Adducin antibody (ab54985) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 81 kDa
    alpha Adducin antibody (ab54985) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.

References for Anti-alpha Adducin antibody (ab54985)

ab54985 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (muscle,rhabdomyosarcoma)
Specification muscle,rhabdomyosarcoma
Preparation Cell harvesting/tissue preparation method: the cell were trypsinized and counted to 5x10^5 cell/ each group
Sample buffer: FACS buffer
Fixation No
Permeabilization No
Gating Strategy the cells were gated on FSC/SSC to remove cell debris
Username

Dr. Chang Chuan-Fa

Verified customer

Submitted Feb 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"