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Rabbit monoclonal [EP2175AY] to alpha Adducin (phospho S59)
ab76251 only detects alpha Adducin when phosphorylated at Serine 59.
, IPmore detailsUnsuitable for:
Flow Cyt,ICC or IHC-P
Synthetic phospho-peptide corresponding to residues surrounding Serine59 of human alpha Adducin
- HeLa cell lysates, untreated, or treated with calyculin A
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/5000. Predicted molecular weight: 81 kDa.
Is unsuitable for Flow Cyt, ICC and IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Belongs to the aldolase class II family. Adducin subfamily.
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Cytoplasm > cytoskeleton. Cell membrane.
Information by UniProt
- ADD A antibody
- ADD1 antibody
- ADDA antibody
Western blot - alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)
All lanes :
Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251) at 1/10000 dilutionLane 1 :
HeLa cell lysate (untreated)Lane 2 :
HeLa cell lysate, treated with calyculin A
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
goat anti-rabbit HRP labelled IgG at 1/1000 dilutionPredicted band size:
81 kDaObserved band size:
115 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"