Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)

Overview

  • Product nameAnti-alpha Adducin (phospho S59) antibody [EP2175AY]
    See all alpha Adducin primary antibodies
  • Description
    Rabbit monoclonal [EP2175AY] to alpha Adducin (phospho S59)
  • Specificityab76251 only detects alpha Adducin when phosphorylated at Serine 59.
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phospho-peptide corresponding to residues surrounding Serine59 of human alpha Adducin

  • Positive control
    • HeLa cell lysates, untreated, or treated with calyculin A
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: 1/40.
    WB: 1/2000 - 1/5000. Predicted molecular weight: 81 kDa.
    Is unsuitable for Flow Cyt, ICC and IHC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMembrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
    • Tissue specificityExpressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
    • Sequence similaritiesBelongs to the aldolase class II family. Adducin subfamily.
    • DomainEach subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
    • Cellular localizationCytoplasm > cytoskeleton. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADD A antibody
      • ADD1 antibody
      • ADDA antibody
      • ADDA_HUMAN antibody
      • Adducin 1 (alpha) antibody
      • Alpha adducin antibody
      • Alpha-adducin antibody
      • Erythrocyte adducin alpha subunit antibody
      • Erythrocyte adducin subunit alpha antibody
      • MGC3339 antibody
      • MGC44427 antibody
      • OTTHUMP00000151163 antibody
      • OTTHUMP00000151164 antibody
      • OTTHUMP00000151165 antibody
      • OTTHUMP00000218664 antibody
      • OTTHUMP00000218665 antibody
      see all

    Anti-alpha Adducin (phospho S59) antibody [EP2175AY] images

    • All lanes : Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251) at 1/10000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate, treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP labelled IgG at 1/1000 dilution

      Predicted band size : 81 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)

    References for Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)

    ab76251 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76251.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"