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Full length native protein (purified) corresponding to Human Alpha B Crystallin.
Our Abpromise guarantee covers the use of ab74441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 20 kDa.|
|ELISA||Use at an assay dependent concentration.|
4% formaldehyde-fixed SK-N-BE cells stained for Alpha B Crystallin (green) using ab74441 at 1/100 dilution in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 min at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60min room temperature, 5min room temperature. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab74441 (D) Composite.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"