Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

Overview

  • Product nameAnti-Alpha B Crystallin antibody [1B6.1-3G4]
    See all Alpha B Crystallin primary antibodies
  • Description
    Mouse monoclonal [1B6.1-3G4] to Alpha B Crystallin
  • SpecificityThere is no reactivity with Beta-crystallin, AlphaA-crystallin, Hsp25, Hsp27 or Hsp47 proteins.
  • Tested applicationsSuitable for: IHC-Fr, WB, ICC, IP, Flow Cyt, IHC-FrFlmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Immunogen

    Full length native protein (Purified AlphaB-crystallin from bovine eye lens).

  • Positive control
    • Recombinant Bovine alpha B Crystallin Protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab13496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-FrFl 1/500.

Target

  • FunctionMay contribute to the transparency and refractive index of the lens.
  • Tissue specificityLens as well as other tissues.
  • Involvement in diseaseDefects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
  • Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
  • Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • AACRYA antibody
    • Alpha B crystallin antibody
    • Alpha crystallin B chain antibody
    • Alpha(B)-crystallin antibody
    • Alpha-crystallin B chain antibody
    • CRYA2 antibody
    • Cryab antibody
    • CRYAB_HUMAN antibody
    • Crystallin alpha B antibody
    • Crystallin alpha polypeptide 2 antibody
    • CTPP2 antibody
    • Heat shock 20 kD like protein antibody
    • Heat shock protein beta 5 antibody
    • Heat shock protein beta-5 antibody
    • HspB5 antibody
    • Renal carcinoma antigen NY REN 27 antibody
    • Renal carcinoma antigen NY-REN-27 antibody
    • Rosenthal fiber component antibody
    see all

Anti-Alpha B Crystallin antibody [1B6.1-3G4] images

  • All lanes : Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : alpha Crystallin
    Lane 3 : alpha A Crystallin
    Lane 4 : alpha B Crystallin
    Lane 5 : beta Crystallin


    Predicted band size : 21 kDa
  • ab13496 staining Alpha B Crystallin in mouse brain tissue by Immunohistochemistry (free floating sections). Samples were incubated with the primary antibody at a 1/500 dilution for 16 hours at 4°C. A goat anti-mouse IgG conjugated to Alexa Fluor®594 was used as secondary antibody at a 1/10000 dilution.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab13496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13496, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemical analysis of murine skeletal muscle tissue, staining Alpha B Crystallin with ab13496. Sections were blocked for 1 hour in 1% BSA and 10% FCS. The primary antibody was conjugated to AlexaFluor®594 before diluting 1/20 and incubating overnight at 4°C.

References for Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

This product has been referenced in:
  • Telkoparan P  et al. Coiled-coil domain containing protein 124 is a novel centrosome and midbody protein that interacts with the Ras-guanine nucleotide exchange factor 1B and is involved in cytokinesis. PLoS One 8:e69289 (2013). ICC/IF ; Rat . Read more (PubMed: 23894443) »
  • Carberry S  et al. Comparative proteomic profiling of soleus, extensor digitorum longus, flexor digitorum brevis and interosseus muscles from the mdx mouse model of Duchenne muscular dystrophy. Int J Mol Med 32:544-56 (2013). Read more (PubMed: 23828267) »

See all 10 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample Rat Tissue sections (brain, 20 micron-thick free-floating sections)
Specification brain, 20 micron-thick free-floating sections
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Marie-Caroline Cotel

Verified customer

Submitted May 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Tissue lysate - whole (muscle)
Total protein in input 20 µg
Specification muscle
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Apr 19 2012

DISCOUNT CODE: *** Expiration date: April 19th, 2012 I am very pleased to hear you would like to accept our offer and test ab13496 in IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an...

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Thanks for your call today and for letting us know about the trouble with ab83339 and ab13496. Please keep me updated about any progress with these antibodies, and let me know if the protocol alterations we discussed are not helpful. Also, we have a fe...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Whole brain homogenate)
Loading amount 10 µg
Specification Whole brain homogenate
Gel Running Conditions Reduced Denaturing (Bis-tris 4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Brian Hitt

Verified customer

Submitted May 07 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Username

Mr. Brian Hitt

Verified customer

Submitted Apr 30 2010

Thank you for your enquiry. Ab13496 has been tested for application in IHC-frozen sections and has not yet been tested in IHC-formalin fixed paraffin embedded sections. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"