Anti-Alpha B Crystallin antibody (ab65940)
Key features and details
- Goat polyclonal to Alpha B Crystallin
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Alpha B Crystallin antibody
See all Alpha B Crystallin primary antibodies -
Description
Goat polyclonal to Alpha B Crystallin -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Pig, Cynomolgus monkey, Orangutan -
Immunogen
Synthetic peptide:
RLEKDRFSVNLD
, from the internal region of human alpha B Crystallin -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 18-19 kDa (predicted molecular weight: 20 kDa).
A 1 hour primary incubation is recommended for this product. Approx 22kDa band observed in Mouse and Rat Eye lysates (calculated MW of 20.1kDa according to Mouse NP_034094.1 and Rat NP_037067.1). |
Notes |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 18-19 kDa (predicted molecular weight: 20 kDa). A 1 hour primary incubation is recommended for this product. Approx 22kDa band observed in Mouse and Rat Eye lysates (calculated MW of 20.1kDa according to Mouse NP_034094.1 and Rat NP_037067.1). |
Target
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Function
May contribute to the transparency and refractive index of the lens. -
Tissue specificity
Lens as well as other tissues. -
Involvement in disease
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients. -
Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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Database links
- Entrez Gene: 396089 Chicken
- Entrez Gene: 281719 Cow
- Entrez Gene: 102128339 Cynomolgus monkey
- Entrez Gene: 12955 Mouse
- Entrez Gene: 100172850 Orangutan
- Entrez Gene: 100127443 Pig
- Entrez Gene: 100009295 Rabbit
- Entrez Gene: 25420 Rat
see all -
Alternative names
- AACRYA antibody
- Alpha B crystallin antibody
- Alpha crystallin B chain antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab65940 has not yet been referenced specifically in any publications.