Recombinant
RabMAb

Anti-Alpha B Crystallin antibody [EPR2751Y] (ab75833)

Overview

  • Product name
    Anti-Alpha B Crystallin antibody [EPR2751Y]
    See all Alpha B Crystallin primary antibodies
  • Description
    Rabbit monoclonal [EPR2751Y] to Alpha B Crystallin
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in human alpha B Crystallin

  • Positive control
    • Human heart cell lysate and human muscle tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab75833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      May contribute to the transparency and refractive index of the lens.
    • Tissue specificity
      Lens as well as other tissues.
    • Involvement in disease
      Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
    • Sequence similarities
      Belongs to the small heat shock protein (HSP20) family.
    • Cellular localization
      Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • AACRYA antibody
      • Alpha B crystallin antibody
      • Alpha crystallin B chain antibody
      • Alpha(B)-crystallin antibody
      • Alpha-crystallin B chain antibody
      • CRYA2 antibody
      • Cryab antibody
      • CRYAB_HUMAN antibody
      • Crystallin alpha B antibody
      • Crystallin alpha polypeptide 2 antibody
      • CTPP2 antibody
      • Heat shock 20 kD like protein antibody
      • Heat shock protein beta 5 antibody
      • Heat shock protein beta-5 antibody
      • HspB5 antibody
      • Renal carcinoma antigen NY REN 27 antibody
      • Renal carcinoma antigen NY-REN-27 antibody
      • Rosenthal fiber component antibody
      see all

    Images

    • Anti-Alpha B Crystallin antibody [EPR2751Y] (ab75833) at 1/2000 dilution + human heart cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 20 kDa
      Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    • ab75833 at 1/100 dilution staining alpha B Crystallin in human muscle tissue, using a HRP/AP polymerized secondary antibody.

    References

    ab75833 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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