Anti-Alpha B Crystallin antibody [EPR2751Y] (ab75833)


  • Product nameAnti-Alpha B Crystallin antibody [EPR2751Y]
    See all Alpha B Crystallin primary antibodies
  • Description
    Rabbit monoclonal [EPR2751Y] to Alpha B Crystallin
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in human alpha B Crystallin

  • Positive control
    • Human heart cell lysate and human muscle tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab75833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionMay contribute to the transparency and refractive index of the lens.
    • Tissue specificityLens as well as other tissues.
    • Involvement in diseaseDefects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
    • Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
    • Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • AACRYA antibody
      • Alpha B crystallin antibody
      • Alpha crystallin B chain antibody
      • Alpha(B)-crystallin antibody
      • Alpha-crystallin B chain antibody
      • CRYA2 antibody
      • Cryab antibody
      • CRYAB_HUMAN antibody
      • Crystallin alpha B antibody
      • Crystallin alpha polypeptide 2 antibody
      • CTPP2 antibody
      • Heat shock 20 kD like protein antibody
      • Heat shock protein beta 5 antibody
      • Heat shock protein beta-5 antibody
      • HspB5 antibody
      • Renal carcinoma antigen NY REN 27 antibody
      • Renal carcinoma antigen NY-REN-27 antibody
      • Rosenthal fiber component antibody
      see all

    Anti-Alpha B Crystallin antibody [EPR2751Y] images

    • Anti-Alpha B Crystallin antibody [EPR2751Y] (ab75833) at 1/2000 dilution + human heart cell lysate at 10 µg

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 20 kDa
      Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    • ab75833 at 1/100 dilution staining alpha B Crystallin in human muscle tissue, using a HRP/AP polymerized secondary antibody.

    References for Anti-Alpha B Crystallin antibody [EPR2751Y] (ab75833)

    ab75833 has not yet been referenced specifically in any publications.

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