Anti-Alpha B Crystallin (phospho S45) antibody (ab5598)

Overview

  • Product name
    Anti-Alpha B Crystallin (phospho S45) antibody
    See all Alpha B Crystallin primary antibodies
  • Description
    Rabbit polyclonal to Alpha B Crystallin (phospho S45)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Sheep, Rabbit, Hamster, Pig, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Alpha B Crystallin aa 44-54.
    Sequence: LSpPFYLRPPSF

  • Positive control
    • Immunofluorescence staining of phospho-alpha-B crystallin (Ser45) in U373 MG cells results in cytoplasmic staining.

Properties

Applications

Our Abpromise guarantee covers the use of ab5598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 8 µg/ml.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 20 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • Western blot of recombinant bovine phospho-alpha-B crystallin (Ser45) using ab5598. Lane 1 is phospho-alpha-B crystallin (Ser45) and lane 2 is unphosphorylated alpha-B crystallin.

References

This product has been referenced in:
  • Kuipers HF  et al. Phosphorylation of aB-crystallin supports reactive astrogliosis in demyelination. Proc Natl Acad Sci U S A 114:E1745-E1754 (2017). Read more (PubMed: 28196893) »
  • Tanito M  et al. Diagnostic Western blot for lens-specific proteins in aqueous fluid after traumatic lens-induced uveitis. Jpn J Ophthalmol 53:436-9 (2009). Read more (PubMed: 19763765) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should yo...

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