Anti-Alpha B Crystallin (phospho S59) antibody (ab103282)

Overview

  • Product name
    Anti-Alpha B Crystallin (phospho S59) antibody
    See all Alpha B Crystallin primary antibodies
  • Description
    Rabbit polyclonal to Alpha B Crystallin (phospho S59)
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Pig, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Alpha B Crystallin, phosphorylated at S59 and phosphorylated at S59.

  • Positive control
    • This antibody gave a positive signal in Human Spinal Cord tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab103282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 20 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    May contribute to the transparency and refractive index of the lens.
  • Tissue specificity
    Lens as well as other tissues.
  • Involvement in disease
    Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
  • Sequence similarities
    Belongs to the small heat shock protein (HSP20) family.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • AACRYA antibody
    • Alpha B crystallin antibody
    • Alpha crystallin B chain antibody
    • Alpha(B)-crystallin antibody
    • Alpha-crystallin B chain antibody
    • CRYA2 antibody
    • Cryab antibody
    • CRYAB_HUMAN antibody
    • Crystallin alpha B antibody
    • Crystallin alpha polypeptide 2 antibody
    • CTPP2 antibody
    • Heat shock 20 kD like protein antibody
    • Heat shock protein beta 5 antibody
    • Heat shock protein beta-5 antibody
    • HspB5 antibody
    • Renal carcinoma antigen NY REN 27 antibody
    • Renal carcinoma antigen NY-REN-27 antibody
    • Rosenthal fiber component antibody
    see all

Images

  • All lanes : Anti-Alpha B Crystallin (phospho S59) antibody (ab103282) at 1 µg/ml

    Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188) with Immunising peptide at 1 µg/ml
    Lane 3 : Human spinal cord tissue lysate - total protein (ab29188) with Non modified peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ab103282 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103282 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab103282 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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